ISSN: 1813-0410

Volume 10, Issue 3

Volume 10, Issue 3, Summer 2014, Page 0-361


Relationship between Bone Morphogenetic Protein15 gene(BMP15) polymorphism and some productive and reproductive trait in Awassi sheep

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 1-17

In this study 50 ewes of native Awassi breed aging (2-5) years and their 62-offspring aging from birth to weaning was used and was carried in the department of animal production / Faculty of Agriculture / University of Baghdad , as well as laboratories of institute of genetic engineering and biotechnology for post graduate studies and over the period from 1/11/2015 to 1/6/2016.
The aim of this study was to investigate the genotypes of Bone Morphogenetic Protein 15 (BMP 15) and their association with the production , growth and reproductive traits . PCR - RFLP analysis of (BMP 15) (153 bp) showed three various genotypes represented as AA , AC , and CC with frequencies 36.00, 40.00 and 24.00 respectively, the frequencies of the A and C alleles were 0.56 and 0.44, respectively, The results showed the effects of the genetic make-up of the BMP 15 gene has a high significant on fecundity ratio while no significant on fecundity rate , there was a variation of high significance of mortality rate in lambs up to weaning, with high significance in the rate of weight-gain variation in lambs from birth up to weaning and chest circumference and height were affected in relation to the variability of BMP 15 genotypes, The results of the current study showed that daily and total milk production of Awassi ewes was significantly increased in relation with variation of BMP 15 genotype in favor of ewes with genotype hybrid AC, moreover a significant variation in length of lactation periods, however, milk components were not significantly affected with variation of BMP 15 genotypes except the percentage of milk fat and in favor of ewes with genotype of AA.

The Study Effect Of Curcumin On Some Of Chemophysiological Parameters And Spinal Cord Tissue In Male Rabbits Subjected To Monosodium Glutamate

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 18-30

This study aimed to know the preventive role of a substance Curcumin . against damage happening in the nervous system and induced textured monosodium glutamate (MSG) in male rabbits. , sixty of adult male rabbits were randomly divided into four equal groups (15 animals / group), the first group (G1) intubated with 1 ml / kg ml / kg of corn oil and served as a control group (G1) . The second group were intubated orally and daily with 3mg /kg of MSG for three months , while the third group (G3) has intubated orally and daily with 60 mg / kg curcumin for three months, the fourth group intubated orally and daily with 3mg /kg of MSG and 60 mg / kg of curcumin for three months .Fasting blood samples were collected from fasted rabbits at pretreated period , Mid-experiment and at the end of experiment to study the following parameters : concentration of Total protein (TP) and Glucose (GLU) in cerebeospinal fluid . The results revealed that oral dosing with MSG caused a significant decrease (P <0.01)in concentration of TP and significant increase (P <0.01) in concentration of GLU compared with the control group. The group that treated with 60mg / kg curcumin revealed a significant increase (P <0.01) in concentration of TP while no significant difference was observed in the concentration of GLU compared with the control group. Experience also shows that oral dosing with MSG and curcumin caused significant decrease (P <0.01) in the concentration TP , and significant increase in the concentration of GLU after end of three months compared with the control group .

Effect of aqueous Extract of Laurus nobilis L. Leafs On Some Physio-logical Parameters and Testis tissue in male white Rabbit Lepus articus.

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 31-41

The Study involved twenty healthy aduly white rabbit males (Lepus arcticus L.). the average weight (1.36-1.54) K.g and age (6-8) months old. The rabbits ware divided to two groups. The first group was involved (10) rabbit that treated with aqueous extract of the laurusnoboilis L. leafes (3ml) subcutaneously the Second group was involved (10) rabbits as control group which treated with distteld water. The study aimed to determine the effect of laurus noboilis L. extract on some blood parameters level of testosterone hormone, sperm count and level of some Ions such as sodium, potassium, phosphorus and calcium. And study the histological structure on testis and mean diameter in seminiferous tubules, spermatogonia, Primary spermatocyte, spermatozoa.

Effect of garlic supplementation on the humoral and cellular immunity after immunization of broilers by sonicated oocysts of Eimeria tenella

Al. H. Awse; I.K. Latif

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 54-62

This study was performed to investigate the effect of garlic with sonicated oocysts immunization of E. tenella on humoral and cellular immune responses in broilers. A total of 150 broiler chicks were divided into 6 equal groups. The 1st and 2nd groups inoculated in ovo with sonicated oosycts of E. tenella and repeated by I/M injection at day 14 .The 3rd and 4th groups injected I/M with sonicated oosycts at day old and repeated at day 14, the 1st and 3rd feeding a basal diet, while the 2nd, 4th and 5th groups feeding basal diet with garlic. The birds of the 6th group fed basal diet and remains as control. All groups were challenged with 50,000 of sporulated oocysts of E. tenella. The results revealed that the immunized groups and immunized with garlic have a significant difference P < 0.05 in the lymphoid organs weight and indices, antibody titers against SRBCs (sheep red blood cells) and Phytohaemagglutinin (PHA) skin test in camper with control and garlic groups specially at day 35. At these instants there was no significance difference between the two procedures of immunization in ovo and I/M.

The Antioxidative Action of Dehydroepandrosterone (DHEA) on the Testicular Histology in adult mice treated with two doses of Nitrofu-rantoin

Mohammed H. Asker; Tahani S.S.Al-Azawi

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 63-71

The antioxidant role of Dehydroepiandrosterone(DHEA) was investigated against two dose of Nitrofurantoin (NFT). Seventy-two adult albino mice were divided in to six equal groups. (G1) control, (G2) received 2 mg/kg B.w of DHEA, (G3) received oral dose of 1.5 mg/kg B.W NFT, (G4) received oral dose of 3 mg/kg B.W of NFT,(G5) received DHEA and 1.5 mg kg/B.w NFT and (G6) received DHEA and 3 mg/kg B.w NFT. Histopathological examination of testes was studied after 30 and 60 day of the experiment and revealed a great number of testicular damage including interstitial hemorrhage and vacuoles, abnormal immature spermatozoa, atrophy in germ cells with severe damage and necrosis of tubules in Nitrofuantoin treated mice. In conclusion, this study showed that NFT has deleterious effect on the reproductive function of male mice and DHEA can protect this system against this harmful effect induced by NFT through its antioxidative activity

Study Effect Of Magnetic Water On Some Physiological Features Of Broiler

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 72-78

The present study included following up the effect of magnetic water influence by 1500 Gause on the number of W.B.Cs ,R.B.C.s , P.C.V. , Hb , Glucose ,cholesterol and the activity of AST and ALT enzymes of 90 Broiler (Ross strain) the variables studied in two period 21 days old and 42 days old. The results show significant increase (p<0.05) with time of cholesterol level and the activity of AST enzyme and significant decrease (p<0.05) in the activity of ALT enzyme. The other values(R.B.Cs, W.B.Cs, Hb ,P.C.V, glucose level) show no significant differences in the two period.

Histological and physiological effects of Sustanon on the liver and Kidneys of male rats

Kareem Hameed Rashid; Ekhlas Abd Hamza

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 1-10

This research on male rats was carried out to investigate the possible effects of three different doses of artificial testosterone (Sustanon) on the histology and function of liver and kidneys of adult male rats. In this experiment 48 adult male rats divided into three groups 1, 2 and 3. These groups were injected with0.05, 0.1, 0.2 mg/kg of sustanon respectively for a period of 42 days. Blood samples were collected at the end of the experiment for the determination of the levels of liver enzymes. Male rats were then dissected out, liver and kidneys were removed, weighed and fixed in 10% formalin for histological study, and 12 adult males were used in parallel to these groups as control group. The results showed that there were significant changes in the levels of liver enzymes in all treated groups in comparison with those of the control. All injected groups showed clear histological changes in the basic structure of liver and kidneys in comparison with those for control group. These changes included the increased number of hepatocytes in liver and also some distortion in renal tubules of the kidneys. Changes like cellular hyperplasia and cell degeneration were also observed in all groups of this study especially those of high doses of sustanon. It was concluded from the results of this study that the injection of sustanon at the doses of 0.05, 0.1 and 0.2 mg/ kg in adult male rats had influenced the histological structure of liver and kidneys and also the levels of liver enzymes.

Epidemiological and Molecular study for Malta Fever

Inas Saad Mohammed

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 11-27

and infection patients via methods of serology (Rose Bengal test) and culture. The PCR assay was investigated as a potential role in detection of some Brucella. Blood samples was used to detection common species like: Brucella melitensis and Brucella abortus from sick persons whom were suspected to be infected with brucellosis.
Blood and serum tests were gotten from individuals whom were suspected of contamination with brucellosis, alluded to numerous doctor's facilities in various city of Baghdad (Karkh and Rusafa parts), which include: (General doctor's facility Mohammad Baqir Al-Hakim, Al-Shaheed Al-Sadder clinic, Al-Imam Ali (rest in peace) doctor's facility), and access to insights and maps in all Iraq areas from Ministry of Health/Communicable Disease Control Center, within the time period of the research that lasted from (March to December of the year 2014) .
A sum of 117 fringe blood tests was acquired from sick persons about whom there were suspects of contamination with brucellosis. The analysis of brucellosis was affirmed by clinical discoveries by utilizing exams of serological nature such as: Rose Bengal test, culture and Gram recoloring and distinctive biochemical exams. To build up a PCR system for conclusion of brucellosis, DNA extraction was done through utilizing a business pack, followed by PCR amplification by using two sets of primers: B4/B5 and IS711 B.melitensis and B.abortus.

Role Of Pomegranate Seed Oil (PSO) Against Hepatotoxicity Induced By Sodium Fluoride In Adult Female Rats (Part II

Ellaf Hussian Ali; Baraa Najim Al-Okaily

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 28-43

This work was designed to evaluate the hepatoprotective effects of pomegranate seed oil (PSO) against toxicity induced by sodium fluoride (SF) in adult female rats. Twenty female rats were divided into four equal group and treated daily for 40 days as follows: Group C administered tap water and served as control , group T1 : received sodium fluoride (120mg/liter) in drinking tap water, group T2: received both sodium fluoride (120mg/liter) in drinking water and administered orally pometone (PSO) 30mg/kg B.W. and group T3 : administered PSO 30mg/kg B.W. orally. Fasting blood samples were collected at 0, 20 and 40 days to estimation of some biochemical parameters and oxidative stress biomarkers . In addition, sections from liver were taken at the end of the experiment for histopathological study. The results revealed that SF treated group caused a significant increase in serum aminotransferases (ALT and AST) activity, total cholesterol ,total bilirubin and peroxynitrite radical concentrations, while GSH concentration was a significantly decrease. PSO caused an alleviation to the hepatic dysfunction induced by sodium fluoride manifested through significant elevation of GSH concentration, in addition, a significant reduction in serum transaminases activity, total cholesterol, peroxynitrite radical and total bilirubin concentrations. In contrast, administration of PSO (group T3) restored almost most of these parameters to near or below to normal levels. Furthermore histopathological examination of liver tissues of group T1 manifested aggregation of mononucleated cells (MNCs), proliferation of hepatocyte, cytoplasmic fat droplet and granulomatous lesion consists of aggregation of macrophage and lymphocyte. All these alteration in liver histology were modified by treatment of rats with PSO. No clear pathological lesion was reported in group T3 received PSO. On conclusion, this study documented the beneficial effect of PSO against the deleterious effects of SF on liver functions of adult female rats.

Fecal pH and fecal score in local cattle

Hasanian Hisham Nasir Al-Autaish; Rahman Kadhum Muhsen

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 44-47

The study was conducted at Thi-Qar province at period from 16-9-2016 to 15-1-2017. Nine hundred ( 900 ) fecal samples were taken rectally from cows. Fecal PH and fecal score were recorded. The results of this study revealed that , the mean of fecal pH and fecal score in our cattle was 7.76 ± 0.086 and 2.72 ± 0.16 respectively . There was no significant differences in fecal pH and fecal score between male and female and between mature and immature cattle ( P > 0.05 ) . There was significant positive correlation between fecal pH and fecal score (P < 0.05 ) .

Effect of garlic supplementation on the humoral and cellular immunity after immunization of broilers by sonicated oocysts of Eimeria tenella

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 48-57

This study was performed to investigate the effect of garlic with sonicated oocysts immunization of E. tenella on humoral and cellular immune responses in broilers. A total of 150 broiler chicks were divided into 6 equal groups. The 1st and 2nd groups inoculated in ovo with sonicated oosycts of E. tenella and repeated by I/M injection at day 14 .The 3rd and 4th groups injected I/M with sonicated oosycts at day old and repeated at day 14, the 1st and 3rd feeding a basal diet, while the 2nd, 4th and 5th groups feeding basal diet with garlic. The birds of the 6th group fed basal diet and remains as control. All groups were challenged with 50,000 of sporulated oocysts of E. tenella. The results revealed that the immunized groups and immunized with garlic have a significant difference P < 0.05 in the lymphoid organs weight and indices, antibody titers against SRBCs (sheep red blood cells) and Phytohaemagglutinin (PHA) skin test in camper with control and garlic groups specially at day 35. At these instants there was no significance difference between the two procedures of immunization in ovo and I/M.

The Antioxidative Action of Dehydroepandrosterone (DHEA) on the Testicular Histology in adult mice treated with two doses of Nitrofu-rantoin

Mohammed H. Asker; Tahani S.S.Al-Azawi

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 58-67

The antioxidant role of Dehydroepiandrosterone(DHEA) was investigated against two dose of Nitrofurantoin (NFT). Seventy-two adult albino mice were divided in to six equal groups. (G1) control, (G2) received 2 mg/kg B.w of DHEA, (G3) received oral dose of 1.5 mg/kg B.W NFT, (G4) received oral dose of 3 mg/kg B.W of NFT,(G5) received DHEA and 1.5 mg kg/B.w NFT and (G6) received DHEA and 3 mg/kg B.w NFT. Histopathological examination of testes was studied after 30 and 60 day of the experiment and revealed a great number of testicular damage including interstitial hemorrhage and vacuoles, abnormal immature spermatozoa, atrophy in germ cells with severe damage and necrosis of tubules in Nitrofuantoin treated mice. In conclusion, this study showed that NFT has deleterious effect on the reproductive function of male mice and DHEA can protect this system against this harmful effect induced by NFT through its antioxidative activity.

Identification of the antibacterial efficacy of ethanolic extracts and oils of some medicinal plants against the growth of Escherichia coli invitro

Jinan abdul-Amir Sabeeh Al-Hussaini

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 68-82

The present study had thrown the light on the in vitro antimicrobial potential of the ethanolic extract of four local medicinal plants; Terminalia chebula, Lawsonia inermis , Origanum vulgare, Thymus vulgaris, and essential oils of five types of medicinal plants; Elettaria cardamomum, Eugenia caryophyllus, Trigonella foenum-graecum, Linum usitatissimum, Brassica nigra against the growth of pathogenic Escherichia coli (E. coli). The antibacterial activity was carried out by using agar well diffusion technique in Mueller-Hinton agar.
The results were obtained by measured the zone of inhibition (mm) around the well that could be exhibited by each plant extract and oil following incubation of bacterial plates and expressed as mean±Standard error (SE). Ethanolic extract of Terminalia chebula was possessed the strongest antibacterial effect among the tested plant extracts, followed by Origanum vulgare and Lawsonia inermis extracts. Escherichia coli was not affected by Thymus vulgaris extract. On the other hand, Escherichia coli was variably susceptible to tow of the used essential plant oils; Elettaria cardamomum and Eugenia caryophyllus, whereas the other three plant oils were not active any more.

Protective Role of Pomegranate peel extract on the Pituitary gland of adult male rabbits treated with CCL4

Wassan M. Hussen; Jawad K. Arrack

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 83-98

This study was carried out to investigate the protective role of pomegranate peel extract for relieving the harmful effects of carbon tetrachloride on the pituitary gland function in adult male rabbits. Twenty four adult male rabbits were used in this study and divided randomly into four equal groups. Rabbits of the 1st group were received 1 ml of orally distal water once a day and olive oil 1/2 ml /kg B.W. intraperitoneal injection [I.P] twice a week as a control group. The second group were treated I.P with 500 mg / kg B.W. of CCL4 mixing with equal volume of olive oil (o.5 ml/kg B.W.) twice a week (group T1). The third group were received orally pomegranate peel extract (100 mg/kg B.W) once a day and olive oil 1/2 ml /kg B.W. I.P twice a week ( group T2). The fourth group were received pomegranate peel extract (100 mg/kg B.W) once a day and treated I.P with 500 mg / kg B.W. of CCL4 mixing with equal volume of olive oil (o.5 ml/kg B.W.) twice a week (group T3). Blood samples were collected at on days (0, 14, 28.42 and 56) for measuring LH, FSH concentrations, total serum protein after treatment. Results showed a significant (P<0.05) decrease in total serum protein in T1 group compared with control group. While T2 group showed a significant (P<0.05) increase in these parameter as compared with the control and T1 group at the end of treatment period. T1 group indicated i a significant (P<0.05) increase in serum LH at(zero and 14 day) and FSH at( from zero time to the 28 day) . These levels, however, were significantly decreased in T1 group compared to what we observed in the control ones at the end of the experiment period. T2 group also showed a significant (P<0.05) increase in serum FSH at (24 day to the 56day of experiment) and LH concentration (at 56 day) compared to control group. Histological study was showed sever congestion of blood vessel and necrotic area in section of anterior pituitary gland in group treated with CCL4, while demonstrate moderate congestion in T3 group.

Comparison the Anti-atherogenic and hypolipidaemic effect of Cod liver oil, pomegranate seed oil and Rosuvastatin in hyperecholestrolemic rats

Karar T. Numaan; Zahraa Z. muslem; Haider H. Humaish

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 99-112

Rosuvastatin known is the more "statin moderating the lipid profile by reduction the low density-lipoprotein than the other statin such as atorvastatin, simvastatin and parvostatin". The present study was designed to investigate the anti-atherogenic and the hypolipidemic effect of cod liver oil, pomegranate seed oil and rosuvastatin in hypercholesterolemic rats. Thirty five albino wister rats were divided randomly into five equal groups, the first group served as negative control, all rats were administrated normal saline. The second group as positive control were rats administrated high cholesterol diet for six weeks. The animals in the third, fourth and fifth groups were administrated for 6 weeks as fallow: cod liver oil ( 0.5 mg/kg rat per day), pomegranate seed oil (200 mg/kg/day) and rosuvastatin (0.5 mg/kg/day, orally), respectively; In addition high cholesterol diet. The result revealed the high cholesterol diet in the positive control group caused a significant (p≤0.05) alteration in the lipid profile by increasing the serum total cholesterol (TC), Low density lipoprotein(LDL-c), very low density lipoprotein (VLDL-c) and triglyceride (TGs) as well as significant (p≤0.05) decrease the High-density lipoprotein. Also, the result showed a significant (p≤0.05) increase atherogenic indices by increase the coronary risk index (TC/HDL-C), atherogenic Coefficient (TC-HDL-C/HDL-C) and Atherogenic index of serum Log(TG/HDL-C) compare with negative control group. Indicated results significant (p>0.05) decrease in the level of serum glutathione (GSH) and significant (p≤0.05) increase in the level of malondialdehyde (MDA). While the animals in the group 3,4 and 5 were administrated CLO, PSO and statin the result clarified a significant (p≤0.05) improvement in studied parameters , through decrease the TC, LDL-C, VLDL-C, TG, MDA and atherogenic indices" , and increase the HDL-C and GSH compared with negative and positive control groups.

An Immune Mediated Pathology And Tissue Immune Responses Induced By Human Tuberculus Cryglobulin In A Murine Models

Jalal A. Al-Azzawi; Ibrahim M S Shnawa; Khalid yassen ALZamily

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 113-124

Serum cryoglobulin were separated , characterized and partially purified from human pulmonary tuberculus patients. Three graded concentrations were made as;0.2,0.3,and 0.4 mg/ml .Four groups of mice, each of five were given 0.25ml. intramuscular in left and 0,25ml., in the right thigh to each animal in each group .the protocol consist of three successive doses from each concentration to each group in a week a part trend followed by one week leave .At the end of the protocol ,animals were sacrificed as well as the tissue blocks were collected, sectioned and processed for staining with H&E by the aim of tracing immune responses at tissue level .The immune response events were found as; Hyper-plasia of showed bronchus associated lymphoid tissue ,hyperplasia of epithelial cells of the bronchus walls, hyperplasia of macrophages in the alveolar spaces and accumulation of macrophages in the bronchial walls of the lungs .Cardiac muscles showed signs of carditis indicating type II hypersensitivity .Kidneys have shown inflammatory events indicating nephritis may be of serum sickness origin and edema can be of Arthus reaction, hypersensitivity type III. Liver sections were showing macrophage between blood vessels as pre-vascular kuff indicating delayed type hypersensitivity type IV. While splenic tissue sections were showing hypoplasia of lymphoid cells in the white pulp and /or de-plition which may be attributed to the action of the regulatory T cells to the white pulp lymphoid cells. The tissue nature do affect the type of response as well as the intensity of the tissue responses were concentration dependent. Human cryoglobulin from tuberculus patients were found pathogenic for mice and the pathology was immune mediated as; Hypersensitivity types II,III, and IV as well as granuloma. The intensity of the responses were concentration dependent.

Hypolipidemic Effect of Apigenin Extracted From Parsley (Petroselinum sativum L.) Leaves In Cadmium Chloride Treated Rats (Part II)

Khatan Ahmed Al-Mezain; Khalisa Khadhim Khudiar; Auos Khahtan Ahmed Al-Mezain

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 125-139

Cadmium has been reported to have cumulative effects on mortality ,cardiovascular , renal and neurologic .flavonoids (apigenin) are naturally occurring phytochemicals possessed divers pharmacological effects hypolipidemic and antioxidant activity .this study was carried out to investigate the protective effect of the flavonoids extracted from (Petroselinum sativum) parsley leaves on some parameters related to cardiac risk in adult male rats exposed to 50 ppb cadmium chloride in drinking water . Crude flavonoids were extracted from parsley leaves . Further purification of flavonoids was performed by gel permeation column chromatography (TLC) . Thirty adult Albino male rats randomly and equally divided into three group (10 rats for each) and were treated daily for 60 days as follows : The rats were kept on ordinary tab water as control (group c) , received drinking water containing 50 ppb of cadmium chloride (group T1) and simultaneously given orally 150mg/kg B.W. of flavonoids (apigenin) extracted from parsley in addition to 50ppb of cadmium chloride in drinking water (group T2). Fasting blood sample were collected by cardiac puncture technique at 0,30 and 60 days of experiment for measuring the flowing parameters : serum lipid profile including: a. triacylglycerol (TAG) .b. serum total cholesterol (TC) .c. serum high density lipoprotein (HDL-C). d. serum low density lipoprotein (LDL-C).e. serum very low density lipoprotein (VLDL-C) concentration .Besides section from heart, aorta were taken at the end of experiment for histopathological study .The results showed that the yield of crude flavonoids from parsley leaves were approximately 2.68% of dray leaves .Purification of crude flavonoids on Sephadex LH-20 clarified three peaks activity and the proportions of the purified fraction P1, P2 and P3 were 14.06, 82.43 and 3.5% respectively. Thin layer chromatography confirmed that p2 was pure apigenin . the result revealed that animal exposed to 50 ppb of cdcl2 water for 60 days caused cardiac damage manifested by a significant elevation in serum TC,TAG,HDL-C,VLDL-C and LDL-C concentrations, with a significant depression in serum HDL-C concentration . Gavage of flavonoids and Cd concurrently caused a significant correction of the previous studies parameters . Histological section of the heart and aorta of cd treated (T1) group revealed an occurrence of early signs of atherosclerotic lesions . Such histological change could not be observed in heart and aorta of animal in group T2 after oral intubation of flavonoids (apigenin). It could be concluded that apigenin is effective in prevention the deleterious effect of cadmium chloride on major rick factors of cardiovascular system.

In vitro antimicrobial studies of new Zn (II) complexes of N- hydroxymethylsaccharin (Sac-CH2OH) and amine ligands

Ahmed S. M. Al-Janabi

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 140-149

A new five Zn(II) complexes of the type [Zn(Sac-CH2O)2(NN)] {where NN = Bipy, Phen, en, dmen} were prepared from the reaction of diamine ligands NN with [Zn(Sac-CH2O)2] in equivalent molar ratio. The prepared complexes have been characterized by IR, 1HNMR, elemental analysis, and molar conductivity.
The antimicrobial studies of the Zn(II) complexes were also tested against Escherichia coli, and Pseudomonas aeruginosa, as Gram-negative; Staphylococcus aureus, Salmonella typhi, and Bacillus subtilis, as Gram-positive bacteria . and results suggested that Zn(II) complexes have significant antimicrobial activity

Iraqi Propolis effective in avoiding deviations of gut rat's homeostasis exposed to AFB-1

Majida A.J.Alqayim; Ahmed Ayed Shehab

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 150-163

Background: Consumption of food contaminated with Aflatoxin-B1(AF-B1), caused deleterious effects on different body’s systems ,specially GIT in direct and indirect manners. To investigate the protective role of propolis against AF-B1 effects on rats gut, 24 male rats divided into equal 4 groups: group (C )was control, second group (AF) received Aflatoxin-B1 ( 0.025mg/kg ), third group( P) received Propolis in dose (50 mg /kg BW) and the fourth group( AFP) received Aflatoxin-B1 (0.025 mg/ 1kg BW) + Propolis (50 mg / 1kg BW).after 60 days of experiment , intestinal D-zylose adsorption and histomorphological changes were measured
Results: Results revealed that intestinal absorption function of D-Xylose sugar was reduced in AFB-1 exposed rats , meanwhile Propolis improve D-xylose absorption in rats exposed to the AFB-1. Histomorphological measurements include mucosa thickness, Villus high, Crypts depth, Villus/crypt ratio, and goblet cells density in the three portions of small intestine decreased significantly in AF group and increased significantly in P group and kept semi-normal in AFP group in comparison with the control group. Analysis of light microscopic photograph revealed that administration of aflatoxin-B1 cause deleterious changes in stomach, and intestinal tissues, while Propolis was efficient in improving these changes to normal condition.

Effect of Coenzyme Q10 on the reproductive system of female rats (Rattus norvegicus ) treated with lead acetate

Shaymaa Zgher Al- Rumaidh; Hayder A. Al-Zamely

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 164-179

This study was conducted to evaluate the effectiveness of the CoQ10 on the reproductive system of female rats and it's ability to reduce the harmful effect of lead acetate. To achieve this goal an experiment was done on 24 mature female rats which are randomly divided into 4 groups, group(1) treated with 1ml/kg corn oil and considered as a negative control group, group(2) treated with CoQ10 at dose 200 mg/kg and considered as a positive control group, group(3) treated with lead acetate at dose 8mg /kg ,and group (4) treated with lead acetate for 30 days then after CoQ10 for another 30 days. Blood samples were taken from all animals after 60 days for biochemical analysis to estimate FSH, LH, estrogen and progesterone hormones .Histological examinations of an ovary and uterus were also involved in this study. Results showed a significant increase (p< 0.05) in serum FSH, estrogen and progesterone levels in group(2) compared to other groups while LH level was significantly increased (p< 0.05) compared to group (3). Group( 3) showed a significant decrease (p< 0.05) in serum FSH, LH level in compared to group (1) and group( 4 ),while progesterone and estrogen levels showed no significant difference (p> 0.05) compared to group(1) . At the same time, there were no significant difference (p> 0.05) in serum LH, estrogen and progesterone levels in group (4) compared to group(1), while there was a significant decrease (p< 0.05) in serum FSH level in group(4) compared group(1). The results of ovary sections ,group( 2) showed normal structure and distribution pattern of various ovary com-ponents, while group (3)showed sever hemorrhage in the ovarian stroma and suppression of ovarian follicles. Group(4) showed particular recovery in ovarian parenchyma. Uterus sections of group(2) showed normal epithelium which lining the uterus and there is destitution uterine glands. Group(3) showed epithelium degenerative, small and non developed uterine glands and hemorrhage in uterine tissue .Other wised uterus in group (4)showed normal columnar epithelium which lining the uterus, and proliferation of uterine glands.

Preventive Role of Olive Oil On Serum Lipid Profile and Antioxidant Status of Methionine Overload Rabbits (Part II)

Wefak Gaborry Al- Bazii; Khalisa Khadim Khudiar

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 180-190

This study was designed to show the possible preventive role of olive oil on serum lipid profile and antioxidant status of methionine overload rabbits. Thirty adult male rabbits were divided into three equal groups (10/group) and treated as follows for eight weeks: first group (GI) was intubated orally with tap water and served as control group, animals in the second group were intubated daily with 100mg/kg B.W. of methionine (GII), while rabbits in the third group were intubated daily with 100mg /kg B.W of methionine for four weeks and then after intubated orally with 0.51 ml /kg B.W/ of olive oil daily for further four weeks (GIII). Fasting blood samples were collected at zero, four and eight weeks of the experiment for measuring serum total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein(LDL-C ),very low density lipoprotein(VLDL-C), high density lipoprotein (HDL-C), glutathione (GSH) and Malondialdehyde (MDA) concentrations .The results of this experiment showed the preventive role of olive oil in alleviation of hyperlipidemia and oxidative stress induced by methionine overload

Eosinophilic gastro-enteritis in a German shepherd dog: Clinical, Haematological and Biochemical study

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 191-201

A 4-year-old male German shepherd dog was presented to the Vet. Clinic / Fac. Vet. Medicine / Dialala University with long past history of anorexia, chronic intermittent vomiting, hematemesis and melena. Clinical examination revealed dehydration, debilitation with generalized paleness of mucous membranes. Blood picture showed anaemia with eosinophilic leukocytosis. Biochemical parameter included mild increase in ALP, ALT and AST enzymes with normal range of creatinine and urea and a decrease in total plasma protein and increase in fibrinogen. There was an excellent animal response to treatment with Predinsolone. Recurrence of clinical signs appeared after stoppage of treatment and the condition get worse. Ultrasonographic examination revealed pathological changes in the liver and stomach of the animal. The animal died after sometime. All previous observations suggested that the case is an advanced fatal eosinophilic gastroenteritis (EG). This is the first report on canine eosinophilic gastroenteritis in Diyala Province/ Iraq.

Toxicopathological Effects of Aluminum Chloride (AlCl3) in Reproductive System of Female Albino Mice

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 202-220

The present study was designed to investigate the toxic effects of aluminum chloride AlCl3 injected intraperitonally of albino female to study the clinical signs and histopathological changes in reproductive system. (159) of Female mice in the age of puberty and weights ranged (30-35) g were used in this study which included three experiments: first experiment: - This experiment was conducted on 87 female mice, divided into 2 groups by intrapertional injection, the first group contained (15) female mice for the purpose of knowing the lowest value and the highest value in the pilot study, while (72) female mice were used for the purpose of determining the dose toxin medium lethal (LD50), and by the way probit method for the purpose of examining the symptoms of acute toxicity , severity , time of appearance and disappearance clinical sings, which estimated 1100 mg / kg of body weight, second experiment: This experiment was conducted on (36) female mice for a period of (14) days, mice were divided into two groups, as follows: 1- sub acute high toxic exposure group: -Injected with I / P high dose(221.83)mg/kg of body weight included 18 females mice 2-Control group: - Injected I / P solution Physiologic solution included (18) female mice. 3rd experiment: -This experiment was conducted on (36) female mice for a period of (60) day of the experiment, they were divided into two groups, as follows: - 1 – Sub chronic low toxic exposure group: injected I / P low dose (55.45) mg/kg of body weight included (18) females mice. 2– Control group: -injected I / P solution Physiologic and included (18) females mice. The study were conducted at days (3, 7, 14) for the sub acute toxic value exposure and at (15, 30, 60) exposure to toxic sub chronic. The study showed that the effects increased with increasing AlCL3dose and duration of exposure. Where results showed clinical signs that were depression of animal, neurological signs, diarrhea, with paralysis and suffocation and ended up comatose and death within half an hour, pathological changes characterized by degeneration and distraction of ovary with oviduct and uterus, necrosis, papillary hyperplasia of endometrial epithelial lining mostly in sub chronic toxic effects at day 60 .

Evaluation of DNA Damage for Some Iraqi Workers Employ in a Wooden Furniture Factories

Wiaam Ahmed Al-Amili

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 221-228

Wood is among the most universe substantial resources and one of the main com-mon kinds of occupational exposure. For this , the research goal was the assess of the genotoxicity effects of wood dust exposure for some Iraqi workers employed in a wooden furniture factories using alkaline comet assay based on measuring the DNA damage that occurred in the white blood cells (WBC) , aimed to minimize the health risks from dangerous substances in the workplace. Fifty workers in a wooden furniture factories and 50 apparently healthy control were used in this study. DNA damage was significantly higher in the wood's workers , than in the control subjects according to the comet parameters. Thus , significantly higher levels of DNA damage observed in wood's workers in whom either smoked ( tail length 40.15 ± 0.54; tail DNA % 32.12 ± 0.87 and tail moment 12.90 ± 0.98) or non-smokers (tail length 36.21 ± 0.43 ; tail DNA % 29.56 ± 0.34 and tail moment 10.70 ± 0.10) than in smoker (tail length 12.81 ± 0.89; tail DNA % 6.60 ± 0.06 and tail moment 0.846 ± 0.02) and non-smokers (tail length 2.63 ± 0.33 ; tail DNA % 2.29 ± 0.07 and tail moment 0.060 ± 0.03) of control group. Present study deduce the relationship of the exposure to wood dust with high level of DNA damage. In conclusion, the results indicated that there was a possibility of using the changes in the level of comet assay as biodosimetry for the detection of DNA damage of workers employed in a wooden furniture factories . Also, the results obtaining was confirmed by usefulness of the alkaline comet assay as a sensitive additional marker in the regular health screening of workers occupationally exposed to dangerous wooden dust

Effect of Diazinon on Reproductive System of Adult Male Mice

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 229-242

This study was designed to investigate the effect of diazinon on reproductive system of adult male mice reproductive performance. Forty adult male albino mice were randomly divided into two equal groups. Collars made to the mice that were caged alone. The animals of the first group were dipped in tap water without of diazinon and served as control group, while the animals of the second group were dipped with diazinon 60% in dilution (1-1000) and used as treated group. Five animals from each group were sacrificed on the days 1, 14, 28 and42 after the end of treated period. The results revealed that there were significant decrease in sperm count, sperm motility, serum testosterone concentration and diameter and thickness of seminiferous tubules. These results associated with significant increase in dead and abnormal sperm. The activity of acetylcholinesterase was significantly decreased at the first day of post treated period. Histopathological examination of the testes also revealed degenerative changes in the cell lining the seminiferous tubules. In conclusion, diazinon had ad-verse effect on the reproductive system of adult male mice may be occurrence of oxidative stress.

The prediction of estrus synchronization in Awassi ewes by vulvar bi-ometry and vaginal cytology

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 243-248

The characteristics of exfoliated vaginal cells and vulvar biometry following estrus synchronization via insertion of vaginal sponges impregnated with 6-Methyl- 17- Acetoxyprogesterone (MAP) were investigated with the aim to predict estrus in ten adult Awassian ewes .The animals were maintained on green and concentrated ration with water ad libitium . All measurements in the ewes were taken before insertion of intravaginal sponges and at 0hrs , 24hrs , 72hrs after removed of intravaginal sponges. Nine out of ten ewes showed estrus behavior in synchronization rate 90%.The results of vaginal cytology showed dominancy of parabasal cells 49.9% before insertion of intravaginal sponges, and dominancy of intermediate cells 62.4% at 0 hour after removed of intravaginal sponges , while at 24hrs and 72 hrs. the dominant cells were nucleated superficial cells and intermediate cells in percentage rate of 44.3% and 41.8% respectively. The results showed that the measurements of vulvar biometry of synchronized ewes during the study period increase in the vertical dimension of vulva significantly (P≤0.05) only after 24hrs of removing of intravaginal sponges

Effects of feeding different levels of ocimum basilicum seeds on per-formance and immune traits of broiler

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 249-258

This study was conducted to evaluate the effect of Ocimum Basilicum seeds supplementation of a commercial broiler diet on the performance and immune response. Three dietary treatments(100 birds/treatment) with two replicates (50 birds/ replicate) one day old straight run Ross 308 . T1 (as control group) birds fed basal diet without any additives. While, T2 and T3 fed diet supplemented daily with 0.3 and 0.6% basil seeds respectively to the end of the experiment (42 days) to investigate the broiler performance and antibody titer against Newcastle disease virus (NDV) and infectious bronchitis (IB).

Histopathological study in Lungs of mice implanted with mammary adenocarcinoma (AM3) and treated with flavonoids (apigenin)

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 259-269

Several studies have demonstrated that the anticarcinogenic properties of apigenin occur through regulation of cellular response to oxidative stress and DNA damage, had a role as anti-oxidant activity and regulate the redox status and prevent damage caused by oxidative stress. The present study aimed to investigate the hitopahtological changes may occurred in pulmonary tissues of mice implanted with mammary adenocarcinoma AM3 and treated with flavonoid. A sixty mice bearing-mice tumor and normal mice divided into G1; (n=3) bearing-AM3 tumor and treated with flavonoids (apigenin) 300mg/kg b.w/orally and daily/30 days, G2; (n=3) mice bearing-tumor only, G3; (n=5) mice treated with flavonoids (apigenin) 300mg/kg b.w/orally /30days, G4; (n=5) as control group treated with D.W. The results of hematoxylene and eosin stained tissues of lung revealed significant histologic changes in tumor-bearing mice of chronic bronchiolitis, interstitial pneumonia and alveolar emphysema, while in treated bearing-mice with flavonoid showed perivascular cuffing, no metastasis seen.

A study the effect of alcoholic extracts of rosemary and pome granate plant (punicagranatum )on bacterial species contaminating imported chicken thighs in a different markets of of Baquba city

Sundus Adil Naji

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 270-279

This study was conducted for isolating and diagnosing bacterial species contaminated imported chicken thighs in different market of Baquba city and measuring the effectiveness of inhibitory of the alcoholic extract of rosemary leaves and pomegranate peel against bacterial isolates the study included a collection of (100) samples of chicken thighs from a number of markets located in different parts of Baquba province .
The result showed contamination 80% of samples while 20% showed no microbial contamination , the results of positive specimens and diagnosis of the use of chemical tests have been shown the highest rate of isolation was 50% for bacteria Salmonella typhimurium while the lowest rate of isolation of bacteria Ps.aeruginosa by 3.75% .

Effect of vitrification on the morphology and viability of early embryos of sheep

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 280-288

This experiments was conducted in the laboratory of High Institute of Infertility Diagnosis and Assisted Reproductive Technologies in Bagdad, to investigate the effects of ultra rapid cryopreservation ( vitrification ) on the normal morphology and viability of ewes early stage embryos . The sheep ovaries were collected from the slaughters ewes immediately after slaughtering ,the immature oocytes were subjected into in vitro maturation(IVM) and in vitro fertilization(IVF) programs .The early embryonic stages were cryopreserved using the vitrification technique, then thawed and assessed from the morphology and viability at two periods : post thawing and 2hr.post thawing. The morphology and viability of early embryonic stages, showed no significant differences in two pronuclear (2PN) at post thawing and 2hr.post thawing periods namely 88.88 and 83.33% respectively. While the 1-2cell showed a higher percentage of normal morphology (93.33%) at the post thawing and 2hr.post thawing periods,also showed a high percentage of normal morphology 3- 4cell embryos at post-thawing and 2hr.post-thawing (86.66%). A high percentage of viable 3-4 cells embryos were obtained at post-thawing (93.33%),which were decreased to 86.67% at 2hr.post-thawing .These results described for the first time in Iraq, using the vitrification technique with 2PN,1-2cells,3-4cells embryonic stages. This will participates in improving the reproductive efficiency of sheep via producing large numbers of embryos which could be used for application of reproductive technologies .

First record of four species of the genus Gyrodactylus Nordmann, 1832 (Monogenea: Gyrodactylidae) from some Iraqi freshwater fishes

Kefah Naser Abdul-Ameer; Fatima Khalaf Atwan

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 289-297

A total of 578 fishes, belonging to 19 species, were collected from Al-Graiat region on the Tigris River at Baghdad province during the period from July 2015 until the end of March 2016. The results showed the occurrence of eight species of the genus Gyrodactylus Nordmann 1832 (Monogenea: Gyrodactylidae). Among these parasites, four species were recorded for the first time in Iraq namely: G. comephori, G. ibragimovi, G. macronychus and G. masu. Also, seven species of fishes were regarded as new hosts for four species of the studied parasites. The description and measurements of these parasites were given as well as their illustrations.

Fish Oil Individual or Combination with L-carnitine on Broiler lipid profile

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 298-306

This experiment was designed to study the influence of fish oil and l- carnitine and their combination on lipid profile of broilers Ross 308 chicks. One hundred fifty straight run 1-day -old chicks were distributed randomly into three equal groups, (50 birds/ treatment) with two replicates as following: 1) birds fed basal diet without any supplementation. 2) birds fed basal diet daily supplemented with 3% fish oil. 3) birds fed basal diet daily supplemented with 3% fish oil plus l-carnitine (50mg per Kg). All chicks received vaccine against Newcastle disease ND strain (B1) by spray at one day of age. Others vaccines of ND strain (Lasota) were administrated by drinking water at the age 10, 20, 30 days respectively. At the end of the experiment, blood samples collected then measured the lipid profile of chicks’ blood serum. The significant improving of triglyceride, cholesterol, and HDL results were observed T3 birds received fish oil 3% and l-carnitine (50mg per Kg) of at age 32 days. Triglyceride, cholesterol, VLDL, and LDL were decreased (P≤0.05) as compared with control. However, HDL was increased (P≤0.05) as compared with control. In conclusion, fish oil plus l-carnitine can be used with broiler ration at a level (3% fish oil and l-carnitine (50mg /Kg) could enhance lipid profile and health status of broilers.

Clinical Isolation and Molecular Diagnosis of Cutaneous Leishmaniasis by using KDNA Gene PCR at some Baghdad Hospitals

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 307-315

This study was aimed to find out the most effective clinical samples and diagnosis methods in chronic cutaneous leishmaniasis (CCL). Smear, aspiration fluid, and filter paper, samples were taken from 44 skin lesions of suspected cases with CCL in some Baghdad hospitals, and they were compared using microscopic examination, culture, and molecular methods.Samples from the clinical patients were examid which showed throug PCR 95.45%(42/44) ,Microscopic examination 72.7%(32/44), culture 27%(12/44).During the study ,products in KDNA –PCR, L.tropica and L. major the observation of expected bands 780 and 650bp,and the filter paper most effective clinical samples from lesion.In conclusion the PCR –based assays tested on our increased the speed and sensitivity of the diagnosis DNA –PCR very important to the species identification ( L. tropica and L. major).

Economic Losses of Condemned Livers and Lungs due to Infectio with Common Reportable Diseases of Slaughtered Ruminants at Kerbala Abattoirs

Hikmat Sahib Al-Nassir

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 316-327

A study was carried out at kerbala slaughterhouses during 6 month-period from October 2015 to March 2016 in order to estimate the economic losses of condemned livers and lungs of slaughtered ruminants due to common reportable diseases. Diagnosis of diseases and lesions of livers and lungs depended on pathological changes of organ, color, size, consistency, presence of parasites and lesions. Out of 67,500 heads of slaughtered sheep, goats, cattle, and buffaloes which were slaughtered during this study at Kerbala abattoirs, condemnation of livers and lungs was carried out in 2002 (2.96%) of the slaughtered animals, of which 22 (0.03%) , 15 (0.02%) , 302 (0.45%) , 91 (0.13%) of the slaughtered animals were due to hydatidosis, facioliasis , lung worms, pneumonia and hepatitis respectively. The highest infection rate of the condemned livers in slaughtered ruminants was recorded due to hydatidosis 1,324(1.96%), followed by hepatitis 91(0.13%) and fascioliasis 22(0.03%). The livers of 1.32% sheep, 7.42% goat, 3.36% cattle and 4.06% buffaloes carried hydatid cysts, and the infection rate of hydatidosis in livers of goats was significantly higher than other animal species with ( P< 0.05 ). However, lungs of slaughtered ruminants were frequently disposed due to hydatid cyst (1.004%) ,followed by pneumonia (0.45%) and lung worms (0.02%), and that lungs of goats (3.72%) were condemned more frequently with a significant difference ( P <0.05) than other animal spp. The findings of the current study revealed that condemnation of livers and lungs of sheep, goat, cattle and buffaloes in kerbala abattoirs due to parasitic infestation and other pathological lesions caused considerable economic losses. The estimated total losses due to condemned livers and lungs in buffaloes and cattle were (28,250,000 Iraqi Dinars ,ID) (21,730 $) but in sheep and goats ,the losses were (46,650,000 ID) (35,884 $) and the total economic losses recorded in this period of study for all species of animals slaughtered in abattoirs due to condemned lungs and livers were (74,900,000 ID) (57,615 $) .

In vitro activity of Triazolo[1,5-a] pyrimidine carboxylic acid extracted from microalgae Hapalosiphon welweschii against the protoscolices of hydatid cyst

Amal KH. Khalaf; Ahmed A. Nasir Al- Amery

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 328-334

The aim of the present study is testing the activity of Triazolo[1,5-a] pyrimidine carboxylic acid extracted from Hapalosiphon welweschii against the hydatid cyst of Echinococcus granulosus. . the results was found that the compound of the methanol extract of Hapalosiphon welweschii is more active by it is explain in vitro activity against the protoscolices of hydatid cyst where the protoscolices are killed after three days – post treatment in concentrations 300 µg / ml followed by 200 µg / ml that explain activity after five days - post treatment in comparison with the third concentration 100 µgml where revealed low activity with the others. GC- Mass spectrum of the methanol extract has explain presence of the compound Triazolo[1,5-a] pyrimidine carboxylic acid in about 13.28 % from the total composition of methanol extract of microalgae H. welweschii

Histological changes of Uterine Horn in the pregnant and non- pregnant Iraqi ewes (Ovis aries).

Muayad Naeem kareem

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 335-346

The present study was designed to describe the histological changes in the uterine horn of pregnant and non- pregnant adult Iraqi ewes. uterine horn samples of Twenty slaughtered ewes were collected from Kerbala abattoir and divided into four groups, group 1 represented non pregnant ewes, group 2 represented pregnant ewes in the early stage of gestation period (20 – 30) days, group 3 represented pregnant ewes in the middle stage of gestation period (60 – 90) days, group 4 represented pregnant ewes in the late stage of gestation period (100 – 130) days, age of the fetus was determined by measuring crown-rump length. The samples were fixed in 10 % formalin, routine histological technique was done and stained with hematoxylin and eosin. Thickness of endometrium of the uterine horn was increased significantly (p ≤ 0.05) in pregnant ewes especially in the middle stage of pregnancy, myometrium significantly decreased ( p ≥ 0.05) in the late stage of pregnancy while there were no significant differences in the perimetrium between pregnant and non- pregnant ewes. The number and the diameter of endometrial glands also showed a significant increase (p ≤ 0.05) in pregnant ewes rather that in non-pregnant one and height of luminal epithe-lium was significantly decreased ( p ≥ 0.05) with the pregnancy advanced, also the glandular epithelium significantly decreased ( p ≥ 0.05) in the middle stage of pregnancy.

Immuno- Genetic Pathological Study of Protozoal Abortion in Aborted Fetus's and Placenta of Iraqi Cattle.

Muna Sachit Hashim; Angham Jasem Mohammed Ali; Taghreed Jabbar Humadai; Eman Hashim Yousif; Thikra Abdulla Mahmood

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 347-353

Current study was conducting on 50 tissues samples which were on 25 placentas from aborted cow and25 from aborted fetuses. Each tissues sample was cutting in to 4 pieces about 1cm; 1st one kept in 10% formaldehyde and sent to pathology laboratory and 2nd kept in clear test tube and sent to microbiology laboratory in Veterinary Medicine College of Baghdad University. Third and four were sent to laboratory of molecular and immunohistochemistry(IHC) to detection DNA defects; P53 (apoptosis factor) and TNF (tumor necrotic factor) a markers on placental and fetal tissues. Further to serological test for hormones in all aborted female. Microbial examination showed positive test for bacterial and protozoa causes. Conclusions pathological findings in aborted fetus mainly were: in brain; neuronal demyelination and in liver; hepatocytes' vacoulation and inflammation and in lung: edematous lesion with inflammation; and in placenta necrotic foci with abscesses. Genetics test showed DNA damage in brain; liver of fetus and placenta. Immunological examination after IHC methods showed that there was detectable increasing in ratio of TNF and P53 in placental and fetal tissues. Serological test showed increase serum level of estrogen hormone and a prolactin hormone.

Effect of adding salvia officinalis oil to the ration in some blood traits of broiler ross 308

Sarmad abdul Razak Alsaadi; Nihad Abdel Latif Ali Alnidawi

journal of kerbala university, 2014, Volume 10, Issue 3, Pages 354-361

This study becomes conducted to analyze the impact of supplementing sage oil to the ration of Ross 308 chicks on some hematological parameters. 117 broiler chicks are used at one day old were randomly occupied to three treatments (by 3 replicates per treatment; 13 chicks per replicate), and treatments were as follows: Control group: without adding Salvia officinalis to the diet, First treatment group: adds Salvia officinalis by 1% and Second treatment group: add Salvia officinalis by 2%. The experiment included a study of the following hematological parameters: Red Blood Corpuscular Count, Packed Cell Volume, Estimation of Hemoglobin and Differential Leukocytes Count. The results improved that the addition of Salvia officinalis by 1 and 2% to broiler diet led to a significant increment (p<0.05) in RBCs count, PCV, and Hb concentration. From other hand, significant decreasing (p<0.05) in H/L Ratio.
The ameliorating that happens in chicks hematological traits attribute to be added Salvia officinalis their ration .