ISSN: 1813-0410

Volume 2, Issue 3

Volume 2, Issue 3, Summer 2006, Page 1-209


Glutathione -S-transferase, Reduce Glutathione and Xanthine Oxidase Activities in Patient with Prostate Cancer

Mufeed J. Ewadh; Khowla A. AL-Shemran; Mohammed A. AL-Khafaji; Moaed O.AL-Gazally; Maha F. AL-Smaisim; Usama F. AL- Jubori

journal of kerbala university, Volume 2, Issue 3, Pages 1-6

In the present study, the estimation of free radicals scavenger enzyme (antioxidant enzymes) such as glutathione-S-transferase (GST) and the reduce glutathione (GSH) activities, in sera with prostate cancer patients (n=25)and healthy control(n=23), were found a highly significant decreased in GSH (p < 0.005 ) and significant decreased GST (p<0.05).Also we determined the activity of xanthine oxidase (XO), and were found elevation and highly significant at(p<0.001) in xanthine oxidase . The correlation between GSH and GST also determined between control and prostate cancer patients, the result show weak positive correlation in comparison with control. Conventional-PAGE profile of crude serum control and prostate cancer for protein show no different between them.

Prolonged Bleeding Time in Uraemia

Riyadh al- Saigh; Fuad Al- Kasab

journal of kerbala university, Volume 2, Issue 3, Pages 7-17

Bleeding is a common and sometime fatal event in uraemic patients. Its etiology is multifactorial.Eighty uremic patients were included in this study. 39 were males and 41 were females. Their ages ranged 18-70 years. They received haemodialysis (HD), peritoneal dialysis (PD) or both. Bleeding time (BT) was measured by intravenous method. BT is considered prolonged if it is > 10 minutes.This study found neither the patients' age nor their sex affect the BT. The effect of duration of the illness on BT is remarkable. HD affects BT while no effect for PD was found.The commonest clinical manifestation of uraemic bleeding tendency is epistaxis.Among the variable characters of uraemic patients that adversely affect BT were raised serum creatinine (S. cr.), low packed cell volume (PCV), and prolonged partial thromboplastin time (PTT).This study recommends measurement of BT as a simple bedside test for uraemic patients to identify the risk of bleeding at early time. Corrections of abnormal variables like:Anemia by eryhtropoiten and/or blood transfusion,azotemia by dialysis,3-DDAVP usage and/or cryoprecipitate for acute bleeding episode,and estrogen therapy and others.Have a good impact on treating bleeding in uraemics.

ESSENTIAL OIL COMPOSITION OF LAVANDULA OFFICINALIS L. GROWN IN JORDAN

Saad A. Ihsan

journal of kerbala university, Volume 2, Issue 3, Pages 81-21

Essential oils isolated by steam distillation from the fresh and dry flowers of Lavandula officinalis L., the oil yields were (1.35% , 3.8%) respectively . Oils were analyzed by GC, among 26 compounds identified , the major components were 1,8 – cineole (18.9% , 20.3%) , linalool (34.2%,33.0%) and borneol (12.1%, 11.0%), respectively .

Thermal Effect on Viscoelastic Stress Analysis for Incompressible Material

Montadher A. Muhammed

journal of kerbala university, Volume 2, Issue 3, Pages 29-38

This research permits to use a numerical method for Viscoelastic stress analysis , by using a three parameter model for linear incompressible material and assuming thermorehologically simple material with lower Poison's ratio. The profit of the method is included in this research using finite element method. Thermal effect is indicated here using WLF equation , and this increase the computational efficiency by taking a large time steps. Examples is indicated and comparison of the results with a software program (NASTRAN) and another references which used another methods for solutions will be done , as well as, the efficiency of the method will be discussed.

Serum Antioxidant Enzymes and Purification of Blood Cells Cu-Zn Superoxide Dismutase in Chronic Childhood Diarrhea

Moaed O. Al-Gazally

journal of kerbala university, Volume 2, Issue 3, Pages 39-51

This study included two parts: the firstly, measurements the antioxidant enzymes activities [Superoxide Dismutase (SOD), Catalase (CAT), Reduce Glutathione (GSH) and Glutathione –S- Transferase (GST)] and lipid peroxidation product (LPO) levels in sera of children healthy[control(n=35),male=16,females=15] and children with chronic diarrhea less than two years[patients (n=41), male=21,females=20].The results revealed that highly significant increasing in the SOD activity and LPO in sera of children with chronic diarrhea (p<0.01) in comparison with that of controls subjects. The results show depletion in the CAT, GSH and GST activities in sera of children with chronic diarrhea (p<0.01) to compared with control group. This increase and decrease in this enzyme in male more than female. The correlation between the level of GSH and SOD, GST.MDA. The results show highly negative correlation (significant) between GSH and (SOD, MDA) while weak positive correlation (no significant) between GSH and (GST, CAT).
The secondly part from this study included the purification of erythrocytes Cu-Zn superoxide dismutase from control and children with chronic diarrhea by ion exchange chromatography, on a column (7×0.7 cm) of DEAE-Sepharose-CL-6B. The purification of SOD had a specific activity of 2956 U/mg protein(control) and 6502.6 U/mg protein for patients, and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS) and each of its to subunit has a molecular weight about[(18500 Daltons, control) , (18300 Daltons, children with chronic diarrhea)].

Alterations of Vitamin "D" level in Sera of Iraqi Population

Ahmed M.Issa; Sana A. Ibraheem

journal of kerbala university, Volume 2, Issue 3, Pages 58-64

Vit D is a steroid aid to maintain the normal level of calcium in the circulation. It was measured by the high performance liquid chromatography technique. This technique was of choice in measuring the low levels of vit D in sera. In the current study 60 person (29 males and 31 females) enrolled to assess the level of this vitamin in their bodies. The main level of vit D was 19.21 ng/ml in both sexes and there are many significant differences between males and females in addition to a noticeable change in vit D level due to seasonal variation during summer and winter. The study emphasized on the importance of sun rays as a necessary tool to enhance the level of vit D and prevent vit D deficiency in both sexes

Study of the Biological Activity of Aqueous Extract of Cuminum cyminum L. and Hibiscus sabdariffa L. and Detection of Some Active Groups in them.

Hussein Kadhem Abdul Hussein; Najeh Hashem Kadhem; Zuhair Hamid Abbod

journal of kerbala university, Volume 2, Issue 3, Pages 65-72

The studies about the effect of plant extract against different types of bacteria are still one of the most important fields of researches because they are available, cheep, safe, and easy to use by not professional populations. In this work, two plant extracts (Hibiscus sabdariffa) and Cuminum cyminum) were tested for there possible biological activity against bacteria. Boiled aqueous extract of both plants at the following concentrations (1, 5, 10, and 20%) were used after cooling. Agar well diffusion was used to examine the biological activity of each extract and the results expressed as zone of inhibition in (mm). The bacteria used in this study are (Gram positive: Streptococcus faecalis and Staphylococcus aureus) and (Gram negative: Pseudomonas pyogens, Escherichia coli, and Klebsiella pneumoniae).The tests for functional groups that can be extracted by water were carried out. Hence Alkaloids, Saponines, Tannins, Glycosides were screened using a suitable method while oils noticed as upper layer (if present). At 1% concentration, there are no detectable inhibition zones of both aqueous extracts. The inhibition zones appeared at 5% and further inhibition showed as concentration increased. In general, the aqueous extract of Hibiscus sabdariffa has shown more biological activity than the corresponding concentration of Cuminum cyminum. The lowest activity was shown against Escherichia coli bacteria while the other bacteria showed different zone diameters as a response for the same concentration. In Cuminum cyminum there are detectable amounts of essential oils, glycosides and high amounts of tannins while alkaloids and saponines are not detectable using the described methods. In Hibiscus sabdariffa, there is a detectable amount of saponines, glycosides, and high amounts of tannins while there are no positive results for essential oils and alkaloids.In conclusion, the aqueous extracts of H. sabdariffa and Cuminum cyminum have antibacterial activity at concentrations ≥5%. Hibiscus sabdariffa has shown more biological activity than the corresponding concentration of Cuminum cyminum. This fact may be due, in part, to the presence of saponines in Hibiscus sabdariffa. The results can be explained through the presence of different active substances in the aqueous extract of both plants.

التقييم المايكروبي لبعض أنواع اللحوم في السوق المحلية لمدينة كربلاء المقدسة ، وتأثير الخزن عليها

KADHEM; NAJEH HASHEM

journal of kerbala university, Volume 2, Issue 3, Pages 73-81

This paper contains a study of the microbial content of the cow carcass and minced meat in butcher shops in Karbala city from 1-April-15May.The study also deals with the influence of normal storage under cold conditions and bad storage (25с° room temperature) in Iraqi current conditions on the development of the number of microbes under study which included: the aerobic plate count (APC), count of Total coliform ( TC.), the number of staphylococcus aureus , the total number of mold & yeast (M &Y). Checking the presence of Salmonella bacteria and also checking the Hydrogen number (PH) For the meat samples. It is noted that more than 66% of the cow carcass and the minced meat samples have shown that their negligence of international properties as regards the (APC) and the (TC).While the number of staphylococcus aureus were unaccepted in 50% and 30% of the cow carcass and minced meat respectively .As regards the molde and yeasts (M&Y), all meat samples were counter to the international properties and that’s the evidence of meat contamination in the external environment. The Salmonella bacteria were present in 66% of the cow carcass and in 33% of the minced meat. There was no significant effect in the increase or reduction of the presence of these bacteria for both types of meat whether storage under 5C° or 25C° .As regards the other microbial numbers , they developed remarkably and signs of perceptible damage for both types as well as storage temperature have been demonstrated and this is owing to the rise of the primary numbers for these tests and also due to the negligence of proper hygienic conditions that slaughterhouses should abide by, the slaughtering outside slaughterhouses, and the absence of hygienic control.

Solution of Fractional Differential Equations by Using Variational Approach

Fadhel S. Fadhel; Basim K. AL-Sultani

journal of kerbala university, Volume 2, Issue 3, Pages 82-89

In this paper, we modify a new approach based on variational techniques for solving fractional differential equations of the form:
y()  F(x, y)
y(1)(x0)  y0,
where 0 <  < 1
This approach has its bases on using Magri’s approach (see [8] )for every linear operator, the results are established using direct Ritz method as well as optimization method to solve these fractional differential equations numerically.

WATER RELATION IN ONIONS: 1- INFLUENCE OF PLANT POPULATIONS AND INTERVAL OF FLOWERING STALK ERADICATIONS ON GROWTH AND YIELD OF GREEN AND DRY BULBS OF ONIONS (Allium cepa L. cv. Local Red) GROWN UNDER RAINFALLS AND SUPPLEMENTARY IRRIGATION

Caser G. Abdel

journal of kerbala university, Volume 2, Issue 3, Pages 105-112

This experiment was carried out during the growing season of 2001-2002 and repeated in 2003-2004 in Mosul to investigate the effects of plant populations and intervals of flowering stalk removal on growth , dry bulbs and green yields of onions grown under irrigated and non-irrigated cultivations. The results of 2001-2002 growing season showed that supplementary irrigated onions significantly increased leaf fresh weight (14.4%), root dry matter percentage (7%), as compared to these of rainfalls. Low onion plant population (18 plants.m‾²) was superior over high onion plant population (36 plants. m‾²), as it substantially increased leaf numbers per plant (27.3%), leaf fresh weight (19.7%), fresh weight of dry bulb (25.9%), and yield of dry bulbs (62%). Moreover, this population significantly exceeded high population in 2003-2004 growing season in leaf fresh weight per plant (27.2%), bulb: leaves ratio (7.7%), and fresh weight of individual plant (6.7%). Weekly eradication of flowering stalks in 2001-2002 growing season appeared to be the most potent treatment, as it highly increased leaf numbers per plant (28.6%), bulb: leaves ratio (219%), bulb fresh weight (265%), yield of dry bulbs (263%), and root fresh weight (15.1%), in relation to that of no flowering stalk elimination which resulted in profound dry bulb yield reductions. On the other hand in 2003-2004 growing season, treatment of no flowering stalk eradication was superior on others, particularly on weekly eradication of flowering stalks, as it significantly increased the plant height (6.9%), leaf fresh weight (52.6%), and green onion yield (26.3%). The highest dry onion bulb yield (3.1 kg.m‾²) and green onion yield (6.64 kg.m‾²) were obtained from supplementary irrigated high onion population of weekly flowering stalk eradication, respectively, during 2001-2002 and 2003-2004 growing seasons.

Glutathione -S-transferase, Reduce Glutathione and Xanthine Oxidase Activities in Patient with Prostate Cancer

Mohammed A. AL-Khafaji; Khowla A. AL-Shemran; Mufeed J. Ewadh

journal of kerbala university, Volume 2, Issue 3, Pages 1-6

In the present study, the estimation of free radicals scavenger enzyme (antioxidant enzymes) such as glutathione-S-transferase (GST) and the reduce glutathione (GSH) activities, in sera with prostate cancer patients (n=25)and healthy control(n=23), were found a highly significant decreased in GSH (p < 0.005 ) and significant decreased GST (p<0.05).Also we determined the activity of xanthine oxidase (XO), and were found elevation and highly significant at(p<0.001) in xanthine oxidase . The correlation between GSH and GST also determined between control and prostate cancer patients, the result show weak positive correlation in comparison with control. Conventional-PAGE profile of crude serum control and prostate cancer for protein show no different between them.

The effect of the odd electron on the IR characteristics (frequency and intensity) of fulvene radical anion. Theoretical study.

A. M. Bashi; J. H. Ali; S. M. Hadowi

journal of kerbala university, Volume 2, Issue 3, Pages 90-97

The IR characteristics (frequency and intensity) of fulvene and its radical anion have been predicted by method based on MINDO/3-FORCES calculation. The changes in the ir characteristics accompanying the conversion of the neutral fulvene into the corresponding fulvene radical anion have been estimated. The calculation predicts, in most significant changes, a decrease in ir frequency of stretching and deformation vibrations in fulvene radical anion and an increase in the corresponding ir intensities. The change is more pronounced in the case of methylene wagging vibration. The calculated decrease in frequency of this mode is 455 cm-1 and the corresponding increase in the ir intensity is about 13.4 times.

Stress hypergly cemia as prognostic criteria in stroke patient

Ala Hussain Haider Al- Hulli; Ahmed Hussain Al-Mialy; Haider Ommran Mossa AL-Kazalli

journal of kerbala university, Volume 2, Issue 3, Pages 98-104

Back ground and purpose
Stress hyperglycemia may associate with increase mortality and poor functional recovery in non-diabetic patients after stroke (1) , and this study was done to clarify this relationship.
2-Objective
1- To study the effect of stress hyperglycemia on prognosis and mortality in stroke patients.
2- To compare the results with normoglycemic patients and diabetic stroke patients.
3- To determine whether this stress hyperglycemia occurs more in infarction or hemorrhagic stroke.
3-Method
The effect of stress hyperglycemia on prognosis and mortality in stroke Patients was studied in (50) patients who were admitted to Merjan Teaching hospital by measuring random blood glucose for each patient and assess the severity of stroke by applying Scandinavian stroke scale (SSS) For each patient also type of stroke whether hemorrhagic or infarction was studied in each patient.
4- Results
A total number of 50 patients were studied, 19 (38%) were normoglycemic, 17 (34%) patients had stress hyperglycemia, and 14 (28%) were diabetic patients.
Group (1)
Normal patients (no history of diabetes and didn’t develop hyperglycemia after stroke).
Group (2)
Stress hyperglycemic patients (no history of diabetes and developed hyperglycemia after stroke).
Group (3)
Diabetic patients (already diabetics before stroke).
(SSS) was highest in group (1) followed by group (3) and was worse in group (2) that was statistically significant.
There was no significant difference in mortality among these groups (P >0.05).
Also there was no significant difference between hemorrhage and infarction among three groups.
Key words: Stroke, hyperglycemia.