Characterization of antibacterial agent produced by Streptomyces spp. isolated from soil samples
journal of kerbala university,
2014, Volume 10, Issue 2, Pages 253-260
AbstractThirty soil samples were collected from Hilla City. Twelve actinomycetes spp. were isolated. Five isolates of Streptomyces spp. were detected according to morphological and biochemical testes. Antibacterial activity of these isolates were tested against S.aureus .Streptomyces spp.4 was most active against S.aureus. Streptomyces spp.4 was selected for isolation of antibacterial agent. Antibacterial agent was active against S.aureus and E.coli with inhibition zone(22,14)mm subsequently. Characterization of antibacterial agent was studied. Antibacterial agent was brown in color and having melting point equals to 110 OC. Thin layer chromatography (TLC) for antibacterial agent showed single spot for agent had RF equals to 0.88. Spectroscopic analysis were made. UV spectrum for antibacterial agent showed that single peak with maximum absorption( λ max) at 289 nm. IR (KBr) (v-, cm-1) spectrum for agent had a peak about 3400 cm-1 which indicate presence of OH group absorption, and a peak appearing at 1635 cm-1 that indicated the presence Carbonyl group(C=O). The peak at 1410 and 1450 indicates to presence of CH3 group. Anther peak at 1076 indicated to presence of carbonyl (C=O) fuction of aster or an amide group. Proton magnetic resonance spectrum (NMR) of antibacterial agent indicated to presence a sharp singlet at δ4 may attributed to OH group. Another singlet at δ1.2 indicated to presence of peripheral CH3 group. Also Singlet at δ2.4 may be assigned to presence of CH2 group surrounded by carbonyl bond (C=O).
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