Study of Toxicity, Immunological and some Cytopathic Effects of Vibrio cholerae supernatant
journal of kerbala university,
2013, Volume 9, Issue 1, Pages 95-100
AbstractCrude Vibrio cholerae extract was produced by centrifugation and filtration then the amount of protein was estimated by Folin- lowry method and was found equal to 550 microgram/ml. Extract activity was set by infant suckling mouse method. Toxicity was determined by measuring LD50 and was found equals to 57microgram/ml. Rabbit Anti-bacterial extract antibodies was titrated by precipitation method and the result showed that the titer is 40. Ouchterlony double immunodifussion test between several dilutions of the crude bacterial extract and rabbit antiserum showed positive results in 1/40 and 1/80 of the extract dilutions. The cytopathic effect of extract on mice hearts was also studied by injecting number of mice with 55 and 27.5 microgram/ml concentration of the crude extract and it showed that it has no certain effect on this organ.
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