Serum Antioxidant Enzymes and Purification of Blood Cells Cu-Zn Superoxide Dismutase in Chronic Childhood Diarrhea
journal of kerbala university,
2006, Volume 2, Issue 3, Pages 39-51
AbstractThis study included two parts: the firstly, measurements the antioxidant enzymes activities [Superoxide Dismutase (SOD), Catalase (CAT), Reduce Glutathione (GSH) and Glutathione –S- Transferase (GST)] and lipid peroxidation product (LPO) levels in sera of children healthy[control(n=35),male=16,females=15] and children with chronic diarrhea less than two years[patients (n=41), male=21,females=20].The results revealed that highly significant increasing in the SOD activity and LPO in sera of children with chronic diarrhea (p<0.01) in comparison with that of controls subjects. The results show depletion in the CAT, GSH and GST activities in sera of children with chronic diarrhea (p<0.01) to compared with control group. This increase and decrease in this enzyme in male more than female. The correlation between the level of GSH and SOD, GST.MDA. The results show highly negative correlation (significant) between GSH and (SOD, MDA) while weak positive correlation (no significant) between GSH and (GST, CAT).
The secondly part from this study included the purification of erythrocytes Cu-Zn superoxide dismutase from control and children with chronic diarrhea by ion exchange chromatography, on a column (7×0.7 cm) of DEAE-Sepharose-CL-6B. The purification of SOD had a specific activity of 2956 U/mg protein(control) and 6502.6 U/mg protein for patients, and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS) and each of its to subunit has a molecular weight about[(18500 Daltons, control) , (18300 Daltons, children with chronic diarrhea)].
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