Bcl 2 Proto – oncogene expression correlate with grading of oral carcinoma.
journal of kerbala university,
2010, Volume 6, Issue 4, Pages 101-109
AbstractIntroduction :- Bcl-2 is known to belong to a family of apoptosis regulatory gene products that may be death antagonists (eg Bcl-2 , Bcl-XL , Mcl-l , Al) or death agonists (eg. Bax , Bak , Bcl-XS , Bad) .
Aims :- To identify Bcl-2 oncogene product expression and its correlation to histological grades of oral carcinoma .
Patients and methods :- Twenty four patients were presented with oral carcinoma , Formalin-fixed paraffin-embedded sections were stained with H and E and immunohistostaining . Primary antibody kit (Bcl-2 onco-protein ready to use) clone 124 Dako corporation .
Results:- Bcl-2 Immunohistochemical expression is confined to the basal cell layers in normal oral mucosa , while Bcl-2 expression in oral carcinoma is peripherally located with in infiltrating tumor cells , which were more intensely stained . Our results reveal that Bcl-2 expression was highly correlated with moderately differentiated (G2) and poorly differentiated (G3) of oral carcinoma and Fischer exact probability test reveals that it is statistically positively significant P=0.0027 .
Discussion Our interpretation may be an over expression of Bcl-2 participates in the differentiation of normal oral keratinocytes , or may be that over expression of Bcl-2 oncogene lead to stop the apoptosis and this will lead to increase the cell proliferation of genetically mutated cells which appear as abnormal architecture of cells and disturb normal histological appearance .
Conclusion :- Bcl-2 expression in normal oral mucosa and oral carcinoma Bcl-2 immunoreactivity correlated with.Bcl-2 expression was highly associated with moderately differentiated (G2) and poorly differentiated (G3) oral carcinoma
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