EFFECT Of SOME AMINO ACIDS, VITAMINS AND PLANT GROWTH REGULATORS ON MAINTENANCE AND LENGTH OF PLANTLETS DERIVED FROM IN VITRO CULTURE OF DATE PALM (Phoenix dactyliferaL.).CV.Barhee
journal of kerbala university,
Volume 4, Issue 3, Pages 54-59
AbstractAn experiment was conducted at Date Palm tissue culture laboratory-Date Palm Research Center-Basrah University to study the effect of some amino acids, vitamins and plant growth regulators on maintenance and length of plantlets derived from tissue culture of date palm (Phoenix dactylifera L.)cv. Barhee. The obtained results showed that, MS medium that contained a combinations of amino acids (100mg/L glutamine,25mg/l proline,25mg/L asparagine and 50mg/L arginine) and vitamins at (2,2,3,1 and 1,2,2,2mg/L) from biotin, pyridoxine, c-Pantothenate and riboflavin in the presence 0.5 mg/L IBA and 0.4 mg/L BA was the best combinations in the maintenance and Length of date palms plantlets. The 3rd and 4th amino acids treatments whth2nd and3rd combination of vitamins in MS medium contains IBA+BA steadily increased the plantlets Length compared with other treatments. In addition, it was noticed that 1st and 2nd treatments of amino acids, gave the lowest values in plantlets Length in MS medium free of growth regulators regardless the combinations of vitamins. The plantlets were grown up best without circumvolution in MS medium supplemented with growth regulators, and ready to be taken for laboratory acclimatizing process.
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