Isolation And Identification Of Candida spp. from Gingivitis and Dental Caries and Study their ability to produce Biofilm
journal of kerbala university,
2017, Volume 13, Issue 4, Pages 105-115
AbstractAtotal of 120 mouth swabs were collected from patients infected with gingivitis and dental caries whom attending to specialist center of dental care in Karbala city . this samples were examined laboratory by direct microscope and culture on sabouraud Dextrose Agar fore identified the presence of yeast in study samples .
The laboratory examination of study samples were appeared positive result in 102 samples(%85) and by physiological and biochemical tests were appeared that Candida albicans (%54.9) were the predominant type followed by C.kefyr(% 25.5), C.glabrata (%13.7) and C.krusei (5.9%). The product of biochemical and physiological test appear C.albicans was alone produced of germ tube in serum and chlamydospores on corn meal agar medium and C.krusei were produse surface growth on the surface of sabouraud broth.
Concerning sex and age of patients , ther is no significant differentiated between male 38.2% and female 61.8% in different age group, and there was no significant difference concerning the residence of the patient such as diabetes, uses antibiotic, smoking ,bregnant, and anemia.
Concerning biofilm production , out of 62(60.8%) isolates of Candida spp. Were positivity and the biofilm production was observed more with C . albicans 48.4% (24 isolates were show weak to moderate positive biofilm but 6 isolates only were appear strong positive ) compared to other Candida spp. %30.6for C. kefyr , %14.5for C.glabrata and %6.5 for C.krusei .
Key words : Candida , Biofilm , Germ tube
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