Effect of vitrification on the morphology and viability of early embryos of sheep
journal of kerbala university,
2014, Volume 10, Issue 3, Pages 280-288
AbstractThis experiments was conducted in the laboratory of High Institute of Infertility Diagnosis and Assisted Reproductive Technologies in Bagdad, to investigate the effects of ultra rapid cryopreservation ( vitrification ) on the normal morphology and viability of ewes early stage embryos . The sheep ovaries were collected from the slaughters ewes immediately after slaughtering ,the immature oocytes were subjected into in vitro maturation(IVM) and in vitro fertilization(IVF) programs .The early embryonic stages were cryopreserved using the vitrification technique, then thawed and assessed from the morphology and viability at two periods : post thawing and 2hr.post thawing. The morphology and viability of early embryonic stages, showed no significant differences in two pronuclear (2PN) at post thawing and 2hr.post thawing periods namely 88.88 and 83.33% respectively. While the 1-2cell showed a higher percentage of normal morphology (93.33%) at the post thawing and 2hr.post thawing periods,also showed a high percentage of normal morphology 3- 4cell embryos at post-thawing and 2hr.post-thawing (86.66%). A high percentage of viable 3-4 cells embryos were obtained at post-thawing (93.33%),which were decreased to 86.67% at 2hr.post-thawing .These results described for the first time in Iraq, using the vitrification technique with 2PN,1-2cells,3-4cells embryonic stages. This will participates in improving the reproductive efficiency of sheep via producing large numbers of embryos which could be used for application of reproductive technologies .
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