Evaluation of some solid and liquid media to screening local bacterial isolates for solubilizing inorganinc phosphate
journal of kerbala university,
2015, Volume 11, Issue 3, Pages 22-29
AbstractThis research aimed to determine the efficiency of some agar media and broth for screening of inorganic phosphate solubilizing bacterial isolates. Sixty bacterial isolates were screened for their ability to solubilize phosphate using five culture agar media, differences were recognized upon agar media in which 50% of total isolates gave halo zone on AYG agar plate after 72 hours while only 26%, 20% and 13% of bacterial isolates solubilized phosphate for PVK, NBRIP and NBRIY respectively. Bromophenol blue dye caused only easer detecting for halo zone without change the percentage of solubilizing bacteria. This research indicated a difference between agar media and broth in which 100% of bacterial isolates gave detectable soluble phosphorous on AYG broth with decreasing in pH of broth which reached to3.6 for KP6.5. Soluble phosphorous determined to 62- 320 ppm for the best solubilizing isolates.
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