ISSN: 1813-0410

Keywords : Rat

Effect of bisphenol-A- on reproductive system of female rats (Rattus Norvegicus)

Qasim Hamadi Abid; Ayyed H. Hassan

journal of kerbala university, Volume 13, Issue 1, Pages 56-62

Bisphenol-A-(BPA) is one of endocrine disruptor substances. The present study was conducted to investigate effect of BPA on reproductive system (reproductive hormones) such as Luteinizing hormone (LH), Follicle stimulating hormone(FSH) and Estradiol (E2)and (reproductive organs) such as ovary and uterus .Twenty four female rats were used and divided into three groups (8 animals for each group) randomly. Animals of first group was negative control group and second group was positive control group received normal saline and corn oil orally respectively, while female rat of third group were received BPA suspended with corn oil (250mg/kg B.W/day) orally as treatment group for 30 days. Results of the present study revealed significant increase (P≤0.05) in serum level of (LH, FSH, and E2) while histopathological changes in ovary include presence cyst-like structure and histopathological changes in uterus including increase in uterine luminal epithelial height and increase in number of uterine glands in the endometrium in group administrated BPA in compared with all other groups

Histological Study on the Effect of Metronidazole (Flagyl®) on the Distribution of Collagen Fibers in the Uterus of the Pregnant

Kadhem M. Haddao; Akram Y. Yasear; Hussain A. Abdullatif

journal of kerbala university, Volume 1, Issue 0, Pages 25-36

To determine the effect of Metronidazole (Flagyl®) on distribution of collagen fibers in the pregnant rat uterus during the implantation period on days 7 and 9 day post coitum (dpc), sixty-four female rats of confirmed pregnancy have been used ,divided into two treated groups (48 rats) received oral dosage commercial Metronidazole (Flagyl®( and one control group (16 rats) received distilled water via the same route. Histological technique was applied for sections taken from implantation sites at days 7 and 9 dpc. The sections were stained in Hematoxylin and Eosin and Gomori's one step-trichrome stains. The results indicates that blastocysts were able to implant successfully and have initiated the implantation reaction and decidualization in the uteri of all rats used. As a normal reaction on day 7 dpc collagen fibers was found to be virtually absent in the decidual tissue of primary decidual zone (PDZ) and secondary decidual zone (SDZ) in contrast to undifferentiated zone (UDZ) in which the fibers was widely distributed.
On day 9 dpc collagen fibers were abundant in the mesometrialdecidual zone (MDZ). Collagen fibers were an important support to the blood vessels in this area which represents a prime route for establishment of nutrient supply through maternal blood vessels supplying the chorioallantoic
placenta of rat. The results have revealed that oral intake of Metronidazole did not interfere with the normal process of implantation of rat blastocyst.