ISSN: 1813-0410

Keywords : Follicles

Studies on Biochemical composition in follicular fluid and blood serum in relation to follicular size in Iraqi she-camels (Camelus dromedaries) during the breeding season

journal of kerbala university, Volume 10, Issue 0, Pages 192-202

This study was aimed to evaluate the biochemical composition of follicular fluid (FF) from different sized follicles and it's relationship with that of blood serum (BS) in she-camels during the breeding season. Ovaries were collected (160 ovaries) from 80 non-pregnant adult females camels (7-14 yr. old) slaughtered at abattoir of province of Karbala during the period between November-2012 to February -2013(The breeding season). The ovaries and blood samples transported to the laboratory within 2 hours post slaughter. FF was aspirated from small (3-10mm) and large (11-20mm) follicles and also the BS separated and both stored at -4ºC for further analysis. The FF and BS samples analyzed by using commercially available kits. The results showed that the mean glucose and cholesterol concentration in FF of large follicles were significantly higher (P<0.05) than in small follicles. The differences in concentration of the mean total protein between small and large follicles was non-significantly. The FF of the mean triglycerides concentration decreased significantly (P<0.05) from small to large follicles. The concentration of the mean glucose, cholesterol, total protein and triglycerides were significantly higher in BS in comparison with in FF. The mean Potassium concentration in FF of small follicles were decreased significantly (P<0.05) than in large follicles, while the differences in the mean calcium and magnesium concentration were non-significantly between small and large follicles. Likewise, the mean sodium concentration was significantly higher (P<0.05) with enlargement of follicular size. The mean potassium concentration in BS was significantly lower (P<0.05) than in FF in small and large follicles, while the mean calcium, magnesium and sodium concentration in BS where significantly higher (P<0.05) than in FF in both categories of follicles. The concentration of the mean estradiol and progesterone in FF of large follicles were higher (P<0.05) than in the small follicles. The concentration of the mean estradiol was higher in BS than in FF, while reverse the concentration of the mean progesterone was lower in BS than in FF.