ISSN: 1813-0410

Keywords : Bacillus subtilis

Purification , characterization of chitinase produced from Bacillus subtilis and inhibitory activity on some fungi

journal of kerbala university, Volume 8, Issue 2, Pages 166-173

Bacillus subtilis with highest production of chitinase was selected among other Bacillus sp. Chitinase was extracted from culture media contain shrimp and fish-shell powder as the major carbon.
Enzyme was purified by anion exchanger (DEAE) and Sephadex G-50 Gel filtration with yield and Purification fold, 33.18% and 11.01 respectively.
Optimum PH for activity and stability was 6,5 and 5.5-8 respectively, The purified enzyme had Mw. 37 Kd. Inhibitory activity was 100% against some molds after incubation for 2h with sterilized chitinase solution (Unit).