ISSN: 1813-0410

Keywords : Phagocytosis

In-vitro study the effects of sickle cell disease on phagocytic activity of white blood cells among adult male in Basra city

Nawal K. Ibrahim; Shaymaa J. Al- Sharrifi; Nehaya M. Al-Aubody; Mustafa Abd-Almaged

journal of kerbala university, Volume 12, Issue 0, Pages 36-41

Background: sickle cell disease (SCD) is an inherited disorder of hemoglobin synthesis that is associated with a significant morbidity and mortality. The patho physiology of the disease involves abnormalities not just in red blood cells but also white blood cells (leukocytes) functions.
Objective: this study was tried to identify the effect of sickle cell disease SCD on the phagocytic activity of non- isolated leukocytes from the whole blood, to mirror the in vivo stimulation of leukocytes and the effect of SCD on their phagocytic activity.
Materials and methods: this study involved (56) adult male, aged (18-30) years, subdivided into two groups; case group that consist of (26) patient with sickle hemoglobin phenotype (SS) in a steady state at the time of the study and control group that consist of (30) person with normal hemoglobin phenotype (AA). Hemoglobin electrophoresis and leukocytes count were estimated for both groups. The samples of blood were prepared for chemiluminescence (CL) measurements. Luminol was used to increase the amount of measurable light emitted due to liberation of oxygen metabolites during phagocytosis.
Results: Show a significant decrease in the phagocytic activity of SCD patients (P ˂ 0.0001) in comparison with normal volunteers

In vitro efficiency of Biosurfactant extracted from Bifidobacterium spp in the phagocytosis process

journal of kerbala university, Volume 12, Issue 0, Pages 304-310

This study was conducted to known the effect of Biosurfactant extracted from Bifidobacterium spp bacterium which isolated from yoghurt and evaluate efficiency of phagocytosis in vitro, in this study was to isolate and diagnose the bacteria Bifidobacterium spp of imported yoghurt products,
A total of 45 samples were randomy collected from deferent imported and domestic dairy products source, 5 (11.11%) positive sample of Bifidobacterium spp were obtained from yoghurt product (Kaller seven dairy product) supplied from Islamic Republic of iran ,several methods were used in the diagnosis of bacterium Represented as microscopic and biochemical tests , in addition its compared with standard isolate.
Biosurfactant was extracted from Bifidobacterium spp bacteria and test its efficacy in efficiency phagocytosis in vitro has reached the percentage of phagocytosis 40.9% for the control group and 64.8% of the experimental group ,because of therapeutic importance of Bifidobacterium spp bacteria and the absence of a local study on the effectiveness of biosurfactant of these bacteria to test the efficiency of phagocytosis in our country this stady was came.