ISSN: 1813-0410

Keywords : Biosurfactant

In vitro efficiency of Biosurfactant extracted from Bifidobacterium spp in the phagocytosis process

journal of kerbala university, Volume 12, Issue 0, Pages 304-310

This study was conducted to known the effect of Biosurfactant extracted from Bifidobacterium spp bacterium which isolated from yoghurt and evaluate efficiency of phagocytosis in vitro, in this study was to isolate and diagnose the bacteria Bifidobacterium spp of imported yoghurt products,
A total of 45 samples were randomy collected from deferent imported and domestic dairy products source, 5 (11.11%) positive sample of Bifidobacterium spp were obtained from yoghurt product (Kaller seven dairy product) supplied from Islamic Republic of iran ,several methods were used in the diagnosis of bacterium Represented as microscopic and biochemical tests , in addition its compared with standard isolate.
Biosurfactant was extracted from Bifidobacterium spp bacteria and test its efficacy in efficiency phagocytosis in vitro has reached the percentage of phagocytosis 40.9% for the control group and 64.8% of the experimental group ,because of therapeutic importance of Bifidobacterium spp bacteria and the absence of a local study on the effectiveness of biosurfactant of these bacteria to test the efficiency of phagocytosis in our country this stady was came.

Study Antibacterial Activity of Lactobacillus brevis Against Escherchia coli and Enterobacter aerogenus Causes Diarrhea Among Children in Holy City of Karbala.

Jannat K. Alkhazraji; Wafaa Sadeq Alwazni; Najeh Hashim Kaduim

journal of kerbala university, Volume 10, Issue 4, Pages 193-202

The study included isolation and identification of pathogenic bacterial which responsible for diarrheal infection to 39 stool samples were collected from children under five years of age suffering from diarrhea attending to education children hospital for of education in the Holy Karbala during the period from May to August 2013, as it was obtained on 39 bacterial isolates from these samples, which have been diagnosed by using differential mediums, biochemical tests and by using Api 20 kits, and appeared that isolates belonging to Escherichia coli (43.58%) and Enterobacter aerogenes (30.76%) are the most common isolates that have been selected to complete the rest of the steps of the study.
A total of ten Lactobacillus spp. isolates have been obtained from different sources such as (Yoghurt ,Whey and Raw milk( ,and only one of them belong to Lactobacillus brevis showed ability to produce the cell free supernatant (biosurfactant) and showed antimicrobial activity against all pathogenic bacterial strains tested .The MIC and Sub-MIC of the cell free supernatant (biosurfactant) extracted from Lactobacillus brevis isolate was determined against studied pathogenic bacteria and the results showed that the MIC of the biosurfactant was 70 mg/ml and Sub-MIC was 60 mg/ml respectively, these results indicates that biosurfactant must be used in high concentration to inhibit the growth of pathogenic bacteria.