Keywords : PCR additives
Effect of Dimethylsulfoxide and Betaine on duplex polymerase chain reaction of human beta-globin gene amplification
journal of kerbala university,
2012, Volume 8, Issue 0, Pages 171-177
A variety of additives and enhancing agents can be included in PCR amplifications to increase yield, specificity and consistency of PCR products. Blood samples were collected from (35) apparently healthy individual for DNA extraction and duplex PCR amplification according to the four different PCR component set up include: 1- standard set up (without PCR additives), 2- standard PCR set up plus 5% DMSO, 3- standard PCR set up plus 1M betaine, and 4- standard PCR set up plus 5% DMSO and 1M betaine. The results revealed that the standard optimization condition of duplex PCR amplification is enough to reveal a good PCR amplification of human beta-globin gene, and the studied PCR additives are useful in improvement of amplification in combination of 5 % DMSO and 1 M betaine.