Keywords : Drug interaction
journal of kerbala university,
Volume 11, Issue 2, Pages 80-89
This work aim to get information about the binding of cephalexin(antibiotics) with albumin, and the influence of the solvent polarity and ionic strength on it by using UV-visb spectrophotometric measurements in phosphate buffer solution pH (7.4), and at three different temperatures, (290, 300, 310)K .The UV absorption shows a change and a shift in the absorbency and a shift in albumin and cephalexin peaks, the two changes are indicative of complex formation. The stoichiometry of the interaction were calculated by the method of continuous variations which was1:1at pH 7.4.The equilibrium constant was calculated at three different temperature (290,300,310)K and the thermodynamic parameter such as ∆G, ∆H and ∆S also calculated. The kinetic studies for this interaction follows first order equation with a rat constant value of 13.8×10-4 min-1.