Keywords : Immune effect
journal of kerbala university,
Volume 6, Issue 3, Pages 44-49
Sixty white males mice involved in this study. The range of their age were 8-12 weeks, divided into four groups treatment, each one included three replicates, each replicate include 5 mice, weighted 22g. Aquatic extract prepared from Nigella sativa seeds. Different doses (0, 200, 300, and 500) mg/kg body weight orally applied for 30 consecutive days. Later the ratio of thymus /body, spleen/body, macrophage activities and delayed hypersensitivity (DTH) were determined by standard methods. The thymus index and the (DTH) significant increase p≤0.05 at the concentration of (300, 500) mg/kg body weight from the aquatic extract of N. sativa seeds, but the spleen index not recorded any significant increase as compared with control. The ability of macrophages to phagocyte and the activity of macrophages revealed significant increase p≤0.05 at all concentration (200, 300, and 500) mg/kg body weight but the best was at 500 mg/kg.
The results of this study showed that the best concentration were (300, 500) mg/kg body weight.