ISSN: 1813-0410

Keywords : Glucose

Effect of different types and concentrations of Sugars and Benzyl adenine ( BA) on the shoot formation of Euphorbia tirucalli L. grown in vitro

journal of kerbala university, Volume 7, Issue 1, Pages 138-143

Three different sugars : sucrose , glucose and mannitol in three concentrations (3,5 and 7)%were examined with or without addition of BA (0.0,1.0,3.0,5.0 or 7.0) mg/L to the MS nutrient medium, on which the single noedes containing lateral buds were cultured .
The results showed that no shoot proliferation without BA treatment ,even when all types of sugars were used at a concentration of 3% or mannitol at a concentration of 5% and 7% .
The highest mean number of shoot (2.36) was found when a combination of 5% glucose +5mg/L BA was used , whilest the shoot elongation has significantly been induced (37.25mm) when a combination of 7% glucose+ 5mg/L BA was used . Mostly no response was found when mannitol used with any combination of BA .