ISSN: 1813-0410

Keywords : SDS

Optimization of a new cloud point extraction procedure for the determination of trace amounts of total Barium (II) in some environmental samples by turbidimetric

Shamaa Ibrahem sead

journal of kerbala university, Volume 12, Issue 1, Pages 45-55

A new, simple, and rapid cloud point extraction (CPE) procedure system, combined with turbidity was created for particular partition, preconcentration, and determination of follow measures of aggregate barium in some ecological specimens. The methodology depends on the complexation of Ba(II) particles with chlorazol clear reagent within the sight of SDS as a non-ionic surfactant. The ideal conditions for the CPE of Ba(II) particles were explored as for a few trial parameters, for example, order addition , pH of the solution, SDS and chlorazol blank concentrations, incubation time and temperature, and effect of intervirrnses and time.
The detection limit for Ba(II) ions taking into account the 8 times the standard deviation of the blanks (N:8) was found to 0.004554μg mL−1, while the relative standard deviation (RSD) was 0.041514 . The proposed strategy was broke down for soil and water tests, which assembled from Environment Iraq , as guaranteed reference materials , were utilized, and spike tests were connected to accept the technique. The technique was connected to some genuine ecological examples to assess their aggregate barium levels. With respect to accomplished information, water and soil of contextual analysis is dirtied

Genetic Discrimination among 21 Corn (Zeay mays L.) Varieties through Total Soluble Seed Protein Profile

Thamer Khadhair Merza; Attyaf Jmeel Thamir; Nidhal Abdul Hussein Al-Badeiry

journal of kerbala university, Volume 10, Issue 0, Pages 168-175

This study was conducted to investigate the ability of total soluble seed protein to discriminate among 21 of maize genotypes through using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). According to SDS analysis , a total of 118 amplified bands were obtained ranging in their molecular size 18-86KDa. Five out of main nine bands were polymorphic and four monomorphic with an average of polymorphism reaches 55.5%.
Phylogenetic tree divided 21 of corn genotypes between two major groups each of them divided in to two subgroups .The first main group included 13 genotypes , while the second main group included eight genotypes .
The ability of protein profile to give a distinctive pattern for a particular genotype could serve in future of studying corn seed response to different abiotic stress and studying genetic diversity .