ISSN: 1813-0410

Keywords : TLC

Production of Levan from Paenibacillus polymyxa in Date Syrup and analyzing of levan composition by TLC

Shamim Naser Radhi; Shatha S. Hasan; Sanaa Burhan alden

journal of kerbala university, 2005, Volume 1, Issue 0, Pages 166-172

Levan, an exopolysaccharide, was produced by Paenibacillus polymyxa and its yield was characterized as a function of concentrations of date syrup and sucrose. The optimum condition for levan production from sucrose was in a concentration of 20% at 37°C, pH 7for 48 hrs and the same conditions for date syrup. Under these conditions in sucrose medium levan production reached to 48 g/L and total carbohydrates were 95.8% while for date syrup was reached to 24g/L and total carbohydrates were 69%. Levan was composed mainly of fructose residues when analyzed by thin layer chromatography (TLC). Date syrup is a cheap substrate widely available in Iraq and has potential for levan production.