ISSN: 1813-0410

Keywords : Chickens

In investigation of the gene responsible for the production of hlyA enzyme and aggA gene in E. coli isolated from the diarrhea of chicken by polymerase chain reaction in the province of Al-Qadisiyah

Jenan Nadhim Sadeq; Mahasen abdul razzaq Khudair; Hiba Turkey Atyia

journal of kerbala university, Volume 14, Issue 1, Pages 279-286

This study aimed to knows the prevalence and occurrence of hlyA gene and aggA in E.coli that taken from clinical Diarrheic in Chickens by using polymerase chain reaction (PCR) technology in AL-Qadisiyah Province , 55 of fecal samples has sent to microbiological lab to make microbiological culture and PCR assay, extraction DNA as the first step, run the extracted samples in thermocycler apparatus by using primers design online as the second step , then immigration the samples by using electrophoresis to read the results .(72.72%) represents percentage of isolated E.coli in our study by use culture methods on MacConkey agar, Eosin methylene blue agar and CHROMagar salmonella, also prevalence of hlyA and aggA gene in present study were (45%) and (37.5%) respectively , the virulence strains have more resistance than non- virulence stains, hlyA gene and aggA gene in E.coli contributes in the pathogenesis of organism.