ISSN: 1813-0410

Keywords : ICP

Determination of Arsenic in Human Scalp Hair and Fingernails of Healthy Individuals Resident in Karbala, Iraq by Using ICP-OES

Joda A. Baker; Fadhil K. Ayad; Sultan A. Maitham

journal of kerbala university, Volume 11, Issue 1, Pages 176-182

The use of unconventional biological materials as biomarkers in trace element studies has increased in terms of published research studies. In this study, human scalp hair and fingernails were used to be a possible biomarker for arsenic in the human body as no study has been published in Karbala yet. Samples were obtained from 56 healthy individuals resident in Karbala, Iraq. The level of arsenic was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). The validity, precision and accuracy of the methodology were evaluated using a “pooled” sample for each media and certified reference materials. The validation methods provided acceptable levels of precision and accuracy with lower range of RSD (1.1%) and acceptable range of elemental recoveries (98.09 %), respectively. The results show that the arsenic levels of study sample are in general agreement with the literature ranges for scalp hair with exception reported for fingernails. It was found that there was no significant difference for arsenic between the two tissues (at P < 0.05). In addition, the value of correlation coefficient (r = 0.804, P = 0.05) was indicated that there is a significant positive correlations for arsenic level between scalp hair and fingernails.

Influence of Gender and Smoking Habit on the Trace Elements Levels of Washed Scalp Hair of a Control Population from Karbala, Iraq

Joda A. Baker; Neil I. Ward

journal of kerbala university, Volume 1, Issue 0, Pages 271-282

Hair samples (n=236) (control or healthy) individuals were collected from Karbala, a city in south-western Iraq. The study population consisted of males (n=196) and females (n=40). All cases were subdivided according to smoking habits (non and active) so as to compare the levels of trace elements in scalp hair in relation to smoking habits. V, Mn, Co, Cu, Zn, Sr and Cd levels in washed scalp hair were measured by inductively coupled plasma mass spectrometry (ICP-MS). The validity and accuracy of the methodology were evaluated by using a certified reference material GBW 09101 Human Scalp Hair with an acceptable range for elemental recoveries ranging from 90 to 107 %. The results obtained showed significantly higher mean level (µg/g dry. weight) of Sr (11.58) in the scalp hair when compared with the reference range values for control or healthy individuals reported in different countries (0.06 – 6.31 µg/g). It was found that the mean values of Sr and Co were significantly higher in females than males, whilst the levels of V, Mn, Cu, Zn and Cd were similar (at a probability level p = 0.05). Hair of smokers contained significantly more V, Sr and Cd than the hair of non-smokers (at P = 0.05). The levels of Mn, Co, Cu and Zn were similar in both sub-groups of smoking activity (at P = 0.05).