Keywords : Lucignen
Spectrophotometric-Continuous Flow Injection Analysis(S-CFIA) For The Determination of Cobalt(Π) Ion in River&Alloy Samples Via The Use of 10,10-Dimethyl 9,9-Diacridinium Dinitrate Salt-Hydrogen Peroxide in Alkaline Medium
journal of kerbala university,
Volume 9, Issue 1, Pages 244-258
A newly spectrophotometric analytical method characterized by it is speed and sensitivity was developed for the determination of Cobalt ions in pure, river water and alloys, using homemade continuous flow injection. The method is based on the oxidation of Lucigenin by hydrogen peroxide in alkaline medium to produced N-methyl acridine which gives with Co(Π) a red-brown color complex(λmax=405nm). Linear dynamic range for the absorbance versus Cobalt(Π) concentration was 0.0-100µg.ml-1, the percentage linearity (r2%) was 99.39%, while the L.O.D (S/N=3) was 1.2ng/sample from the step wise dilution for the minimum concentration of lowest concentration in the linear dynamic ranged of the calibration graph with L.O.Q was 6ng/sample. R.S.D% for the repeatability (n=8) was 0.6% for the determination of Cobalt(Π) with concentration of 10µg.ml-1, through put 40 sample.hr-1, sample volume 150µl. The method was applied successfully for the determination of Cobalt(Π) in different samples. A comparison was made between the newly developed method of analysis(S-CFIA) with classical of analysis using the standard addition method via the use of paired t-test. It shows that there was no significant difference at α=0.05(95% confidence interval) between the two methods. Therefore, the newly developed method can be adopted as an alternative method for the analysis of Cobalt(Π).