ISSN: 1813-0410

Keywords : Vibrio cholerae


The Bactericidal Activity of Gamma ,Beta on Vibrio cholerae resistance to Many of antibiotics (in vitro )

Mohammed Twffeek Abdul-Hussein; Faris Nabeeh Jaafar; Huda Zuheir Majeed; Ashraf Sami Hassan; Anaam Hameed Batah; Hadi Aneed Hmeem

journal of kerbala university, Volume 13, Issue 3, Pages 116-122

This study evaluated the effect of gamma , beta irradiation on Vibrio colerae isolated. The experiment included control (without exposure to irradiation) and 5 replicated doses of each gamma and beta irradiation ,which ranged (1.218×10-4 -0.320µSv) and (63.100 -96.950µSv) respectively. The total effect of gamma and beta irradiation on Vibrio cholerae viability was abrogated at (96.915177 & 63.100 µSv) by 137Cs(1µci) and 137Cs(9µci) respectively, the percentage of killing was highly (84%) and (85%) respectively and the viable cells was fewer than control. Gamma and Beta irradiation is efficient to killing Vibrio cholerae that cause many infection to human and may be cause death. The sensitivity of antibiotics of Vibrio cholerae to ten of antibiotic was tested, the results showed that percent of resistant against V. cholerae to Erythromycin , Methicillin, Ampicillin and Tetracycline was 70%, the percent of resistant to Nalidixic acid was 50%, percent of resistant to Amikacin was 10%, while percent of resistant to Chloramphenicol, Streptomycin, Ciprofloxacin and Norfloxacin was 0%.

Study of Toxicity, Immunological and some Cytopathic Effects of Vibrio cholerae supernatant

Yasir Basim Abdulwahhab Qaddoori

journal of kerbala university, Volume 9, Issue 1, Pages 95-100

Crude Vibrio cholerae extract was produced by centrifugation and filtration then the amount of protein was estimated by Folin- lowry method and was found equal to 550 microgram/ml. Extract activity was set by infant suckling mouse method. Toxicity was determined by measuring LD50 and was found equals to 57microgram/ml. Rabbit Anti-bacterial extract antibodies was titrated by precipitation method and the result showed that the titer is 40. Ouchterlony double immunodifussion test between several dilutions of the crude bacterial extract and rabbit antiserum showed positive results in 1/40 and 1/80 of the extract dilutions. The cytopathic effect of extract on mice hearts was also studied by injecting number of mice with 55 and 27.5 microgram/ml concentration of the crude extract and it showed that it has no certain effect on this organ.