ISSN: 1813-0410

Keywords : Follicular fluid


Relationship of some hormonal and Ionic Constituents of ovarian follicular fluid with size of the follicle in local cows

journal of kerbala university, Volume 11, Issue 1, Pages 73-78

The purpose of this study was to examine the hormonal and ionic constituents of ovarian follicular fluid in relation to follicle size in local cows. The study was conducted at laboratories of department of animal technical production/ Al-Musaib technical collage, from September 2014 to February 2015. The ovaries were collected (120) ovaries from (60) non-pregnant cows which slaughtered at abattoir of province of Karbala. The ovaries were transported to the laboratory within 2hours post slaughter. Follicular fluid was aspirated from small (3-5mm), medium (6-10mm) and large (11-20mm) follicles, and stored at -5 Cº for further analysis. The follicular fluid samples were analyzed using commercial kits. The results show that the follicular progesterone and estradiol hormones concentration were significantly increased (P<0.05) with increase of follicular size. The difference in concentration of sodium between small and large follicles was non-significant, while was significantly higher (P<0.05) in large follicles. The difference in concentration of magnesium among three categories of follicles were non-significant. The results of the present of the study also indicated the concentration of calcium was significantly higher with increased of follicular size, while reverse the concentration of potassium was significantly lower (P<0.05) with enlargement of follicular size.

Studies on some Metabolites, Ionic and Hormonal Composition in Ovarian Follicular Fluid and Blood Serum in relation to size of the Follicle in Iraqi Buffaloes

H.M. Al-Rubaeae

journal of kerbala university, Volume 11, Issue 0, Pages 253-263

The objective of this study was to determine the biochemical composition of follicular fluid obtained from different sized follicles and it's relationship with that of blood serum in Iraqi buffaloes. Ovaries of adult non-pregnancy and healthy buffaloes were collected from each buffalo at the time of slaughter. Follicular fluid was aspirated from small (3-10mm) and large (11-20mm) follicles and also serum separated and both stored at -20ºC for further analysis. The follicular fluid and blood serum sample were analyzed using commercial kits. The results showed that the follicular total protein concentration difference between small and large follicles was non-significant. The follicular glucose concentration was significantly increased (p<0.05) from small to large follicles. The follicular fluid concentration of total cholesterol and triglycerides, were significantly decreased (p<0.05) from small to large follicles. The blood serum concentrations of the total protein, glucose and total cholesterol were significantly higher (p<0.05) than follicular fluid in the small and large follicles, while blood serum triglycerides concentration was significantly lower (p<0.05) than follicular fluid in both classes of follicles. The follicular fluid calcium and sodium concentration were significantly increased with enlargement of follicular size. The follicular fluid potassium concentration of small follicles was significantly decreased than in large follicles, while no difference were found in concentration of magnesium between small and large follicles. The blood serum calcium, sodium and magnesium concentrations were significantly higher in comparison within follicular fluid, while the blood serum potassium concentration was significantly lower than follicular fluid in small and large follicles. The follicular fluid estradiol and progesterone concentrations were significantly increased with increased of follicular size while the blood serum estradiol and progesterone concentrations were significantly lower than follicular fluid in small and large follicles.

Relationship some biochemical and Ionic composition of follicular fluid with ovarian follicular size in local she-camels (Camelus dromedarius)

journal of kerbala university, Volume 9, Issue 2, Pages 123-128

This project was designed to study some metabolites and ionic composition of ovarian follicular fluid and to relate possible changes in composition to follicular size.
Ovaries were collected (120ovary) from 60 adult females camels (5-10 yr. old) slaughtered at slaughterhouse of province of Karbala in breeding season (winter). The ovaries were transported to the laboratory within 2 hours post slaughter. The follicular fluid was aspirated from small follicles (4-9mm) and large follicle (10-20mm).
The follicular fluid was stored at -4 Cº prior to assay. Follicular fluid samples were analyzed for metabolites composition (glucose, total protein and cholesterol), and ionic composition (sodium, calcium and potassium). The results showed that the mean glucose and cholesterol concentration were increased significantly (P<0.05) with increased of follicular size, while the mean total protein concentration decreased significantly with inlargement of follicular size. Likewise, the mean sodium and calcium ions concentration increased significantly with changes of follicular size, while the mean potassium ion concentration decreased significantly with increased of follicular size.