Author : Abid-Ali Abdel-Hussein, Muslim
journal of kerbala university,
Volume 1, Issue 1, Pages 178-185
This experiment was conducted at Plant Tissue Culture Laboratory / Agricultural and Biological Research Center / raqi Atomic Energy Commission during 2001-2003 to study the possibility of using in vitro shoot culture in rooting stage to evaluate salt tolerance of two apple rootstocks (MM106 and Omara).Single shoots were cultured on MS medium as a rooting media supplemented with 0, 20, 40, 60, 80 and 100mM NaCl for 8 weeks.
All rooting parameters (rooting percentage, roots number, roots length )and plantlets growth(height of rooted shoot ) decreased as salt level increased in culture medium, with the reductions generally greater for Omara than MM106 rootstock. Also NaCl effect resulted in plantlet necrosis and a reduction in total chlorophyll content of both rootstocks.owever, plantlets of MM106 showed less relative root and shoot growth reduction under salt stress compared with Omara, therefore, it appeared to be more salt tolerant in vitro than Omara.