ISSN: 1813-0410

Author : Abdulkadhm AL-Ghanimi, Ali

Effect of cultural conditions on the production of glucoamylase from Aspergillus ustus

Ali Abdulkadhm AL-Ghanimi; Aziz Yasir AL-Ethari; Saif Dawood AL-Ahmar

journal of kerbala university, Volume 2, Issue 4, Pages 61-68

A strain of Aspergillus ustus was able to produce glucoamylase in a medium composed of date juice as the main carbon source supplemented with maltose as an inducer and additional carbon source, NH4CL or (NH4)2SO4 as a nitrogen source and KH2PO4 and MgSO4.7H2O as nutrients. The effect of cultural conditions on the glucoamylase production was studied using this medium.Results revealed that date juice of 0.1% total carbohydrates, maltose 1.5% (W/V), NH4Cl 0.2% supplemented with the above mentioned nutrients, adjusted at pH 4.5-5.0 and incubated at 30 ºC for 72 hrs under static conditions was the most efficient and gave higher enzyme yield.