ISSN: 1813-0410

Author : M.Sc,

Modified Method for Preparation of Aurintricarboxylic Acid and Prepare of it’s Chromium (III) Complex: Study its Interaction with Some Human Serum Proteins.

M.Sc; Haitham Delol

journal of kerbala university, 2007, Volume 3, Issue 2, Pages 65-76

This study involves the use of a modified method for preparation of aurintricarboxylic acid (ATA) and use this acid as a ligand for preparation of complex with chromium (III) ion. The prepared complex was studied using different spectrophotometric methods including, UV-Visible spectrophotometry and IR, in addition to molar conductivity. The molar ratio of ligand-cation was found to be (1:2). The complex showed an octahedral geometric structure. The interaction between the formed complex with six blood components including serum total protein (STP), albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), acid phosphatase (ACP), and alkaline phosphatase (ALP) were studied. The measurement occurred before adsorption and after one hour of incubation with the complex. The quantity of the substance adsorbed on complex obtained from the difference in the concentration before and after adsorption per weight of complex. The results showed that the Cr(III)-ATA complex has surface activity and able to adsorb the serum proteins in different capacities according to the following order of quantity adsorpe:
S.AST> S.Albumin> S.T.ACP > S.T.Protein > S.ALP>S.ALT