Author : Hassan Abbas, Zeena
journal of kerbala university,
2008, Volume 4, Issue 2, Pages 164-170
Study design and objective: This is an in vitro experimental study. It was performed to show the effect of gamma radiation on human peripheral blood mononuclear cells (PBMCs) compared with that of ordinary light. Method: First of all, PBMCs were separated and incubated in specialized culture medium to form a cell pool. Tow sample groups were prepared; one containing phytohemaggluttinin (PHA) from which three subgroups; A, B and C each with 30 samples were prepared. The effect of gamma radiation on PBMCs (group A samples) was tested against that of ordinary light (group B) and a control (group C). Another set of samples was prepared similarly, but without PHA. These two groups were incubated under standardized conditions (37°C and 5% CO2). Wet film for direct cell counting 24 hourly and Gemsa staining technique examined by inverted microscope to predict the growth curve, cell count and structural changes induced by irradiation. Results: There was a significant reduction in PBMCs counts in group A, compared to group B and C samples. Furthermore, the growth curve showed prolonged lag phase and delayed and shortened log phase in group A samples. In addition, there were more PBMCs structural changes including anisocytosis, ghost cells and abnormal mitotic figures. These finding were restricted to PHA-containing samples rather than the PHA-free set. Conclusion: The effect of gamma irradiation, represented by reduction in cell count and structural abnormalities, in vitro, was shown to be specific for activated PBMCs (i.e) exhibited on the actively dividing mononuclear cells.