ISSN: 1813-0410

Author : Ebraheem Sa, Shaima


Determination of some chemical compounds and the effect of oil extract from orange peel on some pathogens

Narjis Hadi Mansoor Al-Saadi; Najwa Shihab Ahmad; eed; Shaima Ebraheem Sa

journal of kerbala university, Volume 4, Issue 4, Pages 33-39

This research involves detection of the chemical constituents of orange peel; it contains alkaloids, saponins, terpenes, resins, flavonoids, phenols, tannins, and sugars but not contains coumarins and steroids. In addition sugar, proteins, moisture and ash were determined and their percentage were (23.8%, 4%, 11.86%, 5.34%) respectively. Oil of orange peels was extracted and its percentage was 13.12% then its anti-microbial activity was tested against micropathogen that include Aeromonas hydrophila, Klebsiella pneumonia, Psdumonas aeruginosa, Ecsherichia coli, Psudomonas fluorescenes, Proteus spp. Enterococcus feacalis, Staphylococcus aureus, Candidia albicans and Listeria spp. using agar diffusion wells. Different concentrations of oil were prepared (12.5, 25, 50 and 100,) mg/ml and the diameter of inhibition zone were measured. Oil extract was more effective against Aeromonas hydrophil and Klebsiella pneumonia than other pathogens were tested. In addition some elements were determined such as (Fe, Mn, Zn, Ni, Cu, Cr, Pb, Cd and P). The results demonstrated that orange peels contain these elements at concentrations (125, 88, 13, 1.6, 1.3, 1.2, 0.25, and 0.11) µg/ml. and 0.2% of phosphorus.