ISSN: 1813-0410

Author : Ahmed Abid, Salam

Spectrophotometric determination of adrenaline in pharmaceutical preparations using Prussian blue reaction

Mohammed Jassim Hamzah; Abdul Barry Mahdi Mahood; Salam Ahmed Abid

journal of kerbala university, 2008, Volume 4, Issue 4, Pages 9-14

A simple ,sensitive and accurate spectrophotomeric method for the determination of adrenaline in pharmaceutical dosage forms has been developed. The method is based on the reaction of adrenaline with Fe+3 ion to produce Fe+2 which is upon further reaction with Potassium hexacynoferrate(III) to produce a Prussian blue color product that has a maximum absorption at λmax=700nm.A graph of absorbance versus concentration shows that beer law is obeyed over the range 0.1-2.0 p.p.m with a molar absorpitivity of ( 7.13x104 ,a sandall sensitivity of( 2.56x10-6 µ ,correlation coefficient of 0.9990 , a relative standard deviation of ( 0.12-1.36%) and detection limit of 0.017µ depending on the concentration. The optimum conditions for full color development are described and the proposed method was applied satisfactorily to pharmaceutical preparations containing adrenaline.