journal of kerbala university

The objective of this study was the extraction of Lectin from mushroom Agaricus bisporus (ABL) and concentrated using precipitation in Ammonium sulfate from 25-70% saturation, followed by dialysis against distilled water and PBS, and lyophilized. The cytotoxic effect of five concentration from 312.5-5000 µg/ml of ABL were studied in cytotoxicity assay ( in vitro ) of two tumor cell lines, Human epithelial cell carcinoma of larynx (Hep-2) and Murine mammary gland carcinoma (AMN3) at different exposure times 24, 48 and 72hr. The results revealed high significant differences (P ≤ 0.001) in inhibition rate (IR) of Hep-2 and AMN-3 dependent on Lectin concentration and exposure times, IR were the higher in Hep-2 at 2500 and 5000 µg/ml of Agaricus bisporus lectin (ABL) 24.5 %, 37.5% , and 37.79% , 54.72% in AMN-3 after exposure to 48 hours. The determination of apoptotic cells of Hep-2 and AMN-3 at at the highest concentration (5000) µg/ml ABL revealed significant apoptotic effect in each of (Hep-2) cell line 75.14% and (AMN3) cell line 82.6% in comparison with those of non treated (11.35, 10.82%).
The combination effects of ABL ranged from 10-60 µg/ml with drugs Doxorubicin ( DOX) and Mytomycin-C ( MMC) caused increasing in Cytotoxic effect of DOX to 2.44 time in Hep-2 pretreated with 40 µg/ml ABL and 10 µg/ml DOX and 1.5 time in AMN3 pretreated with 40 µg/ml ABL and 15 µg/ml DOX. But there were no positive effects in Cytotoxicity of MMC on Hep-2 pretreated with ABL, while Cytotoxic effect was increased 2.79 time in AMN-3 cells pretreated with ABL 40 µg/ml and 5 µg/ml MMC.