ISSN: 1813-0410

Author : Akram Hatif, Saad

Comparative study of DNA extraction from semen of cauda epididymis in Ram and Bull

Saad Akram Hatif; Sadeq Jaafer Zalzala

journal of kerbala university, Volume 6, Issue 4, Pages 139-142

This study included semen collection from 20 adult rams and 20 bulls, via aspiration from tail of epididymis. All semen samples subjected to sperm count after staining with eosin. The high number of sperm count in rams show 1180000 sperm/ml, while the lower sperm count reached 540000 sperm/ml, the mean of total samples 1139000 sperm/ml .The maximum sperm count of bulls samples gives 990000 sperm/ml while the minimum appeared 744000 sperm/ml and the mean 953000 sperm/ml. Lysis buffer used for DNA extraction from semen and spectrophotometer in order to assessment concentration of DNA samples, the concentration of DNA in rams and in bulls semen gives 3.5908g/l and 4.4319 g/l. We are concluded that the mean of sperm count in the tail of epididymis in ram more than in the bull ,and DNA concentration in ram samples less than in bull