ISSN: 1813-0410

Author : A.Z. altaher, Wesam

Isolation and characterization of two thermotolerant Kerosene degrading bacteria from oily contaminated soil

Wesam A.Z. altaher

journal of kerbala university, 2012, Volume 8, Issue 1, Pages 188-196

Seven bacterial isolates were isolated from soils contaminated with used diesel engine oils which had been used at a high temperature. Two isolates were gram negative bacteria and the other five were gram positive. All of these bacteria were tested for growth in Bushnell & Haas broth (BH broth) with kerosene as a carbon source at various temperatures (25˚ – 48˚C). One isolate grew well and gave significant growth at (37˚ – 48˚C (, another isolate grew well at ) 37˚ - 42˚C(, the other five isolates lost viability after the first day of incubation at all temperature conditions tested. The two isolates which could grow in BH broth with kerosene were classified as Sphingomonas spiritivorum and Pseudomonas chlororaphis