Author : Hussein Kubba, Rasha
Mutagenic and anti-mutagenic effect of alcoholic extract of Citrullius colocynthis seeds using bacterial system (G-system)
journal of kerbala university,
2012, Volume 8, Issue 2, Pages 229-236
Methanolic extract (80% methanol) of Citrullius colocynthis dried seeds was evaluated for its mutagenic and anti-mutagenic effect in vitro using bacterial system (G-system). The study depended on recording survival index (Sx) as an indicator for study the cytotoxicity effects and the induction of Streptomycin and Rifampicin resistance mutants as a genetic marker.Alcoholic extract of 80% methanol was prepared from dried seeds, and then the extract was dried, then 5 ml of distilled water was used to dissolve the powder, gradual concentrations of plant seeds alcoholic extracts were used to choose the suitable concentration which is resemble the negative control.
The interactions included three types of treatments (pre-mutant, with-mutant and post-mutant) between optimum methanolic extract of dried seed (125 μg/ml)and optimum concentration of methotrexate drug (MTX) (50 μg/ml)as a chemical mutagen and the optimum exposure time of ultra violet ray (UV) (10 minutes, 254 nanometer) as a physical mutagen in order to determine the mechanisms of this plant extract in preventing or reducing the genotoxic effect of MTX and UV.
The results showed that a significant increasing in survival index(Sx) of treated cells which is resemble the negative control when compared with positive control, and the methanolic seed extract of C. colocynthishad no effect to induce resistance mutant for the two antibiotics for all treatment. So these findings suggest that the plant extract have no genotoxic effect on the cells system.
The results of interaction between optimum concentration for extract and the treatment with mutagen to induce resistance mutant for streptomycin and rifampicin found that the MTX had no effect to induce resistance mutant for these two antibiotics, for the three types of treatment (pre-MTX, with-MTX, and post-MTX), while the result of interaction between optimum concentration of extract and the treatment with UV shows that UV had no effect to induce resistance mutant for these two antibiotics, for the two types of treatment (with-UV, and post-UV), the alcoholic extract suppresses or repair mutant from the treatment with MTX and UV ray and give protection 100% for bacterial cells, while the percentage of pre-UV treatment was (90%).