Author : F. Al-rubaei, Abeer
Study of lymphocyte transformation response (LyTR) by using PHA, Con A and Pokeweed mitogens in children infected with Norovirus
journal of kerbala university,
Volume 8, Issue 4, Pages 45-49
There are several agents known to be capable of inducing blast transformation of small lymphocytes. The mitogens phytohaemagglutinin (PHA), concanavalin A (con A) and pokeweed mitogen (PWM) are good examples of such agents . In this study we used The heparinized blood from each of the 45 sample was examined for immuno-suppressive activity.
peripheral blood lymphocytes stimulated with phytohemagglutin mitogen ( 100 ,150,250 µg/ml) , Concanavalin A mitogen (150 µg/ml) and Pokeweed mitogen PWM (150,300 µg/ml ) were severely reduced in acute infantile diarrhea with Norovirus Infection in comparing with acute infantile diarrhea without Norovirus Infection and control group . acute phase heparinized blood appears to block initial steps in lymphocyte transformation.