Author : Gehad Humadi, Hamid
Qualitative and Quantitative screening of alkaline protease production from some pathogenic bacteria
journal of kerbala university,
2013, Volume 9, Issue 1, Pages 309-314
Four different isolates were isolated from different sites of infection, these include 2 isolates of G +ve bacteria ( Staphylococcus aureus from urine and Bacillus cereus from tooth) and G-ve bacteria ( Pseudomonas aeruginosa from burns and Klebsiella pneumoniae from sputum).To detect the ability of these bacteria to produce alkaline protease enzyme in one hand and to correlate the production yield to site of infection from other side, it had been performed the qualitative screening on solid media by using skim milk agar and quantitative screening by measuring the specific activity of each isolates.The results of qualitative screening showed the inhibition zone of Staph aureusand Bacillus cereus were higher than Pseudomonas aeruginosa and Klebsiella pneumoniae (2.8 , 2.1 , 1.3 and 0.5 cm respectively). When these results compared with quantitative results , it had nearly same, in which specific activity of Staph aureus and Bacillus cereus were higher than Pseudomonas aeruginosa and Klebsiella pneumoniae (1955.7 ,1902.8 , 100.8 and 65.4)U/mg respectively.