Author : F. Khudhair, Ahmed
Determination of ciprofloxacin -HCl in pharmaceutical formulations by continuous flow injection analysis via turbidimetric (T180o) and scattered light effect at two opposite position (2N90o ) using Ayah 4SW-3D-T180 -2N90 -Solar - CFI Analyser
journal of kerbala university,
Volume 9, Issue 2, Pages 153-169
A simple and highly sensitive method for the determination of ciprofloxacin in pure and pharmaceutical preparation were developed by coupling the continuous flow injection analysis via turbidimetric (T180o) and scattered light effect at two opposite position (2N90o). Where it is based upon the formation of a yellowish –white precipitate for ion pair compound by using potassium hexacyanoferrate(III) in aqueous medium. The precipitate is measured via the attenuation of incident light and it’s scattering in two opposite directions. Chemical and physical parameters were studied. The linearity of ciprofloxacin is ranged from 1 to 20 mmol.L-1, with correlation coefficient r=0.9927, limit of detection (LOD) 0.55 mmol.L-1(3SB)(S/N=3) for n=15 and the relative standard deviation for 4 mmol.L-1 ciprofloxacin solution is lower than 2% (n=6). Three pharmaceutical drugs were used as an application for the determination of ciprofloxacin. A comparisons were made between the newly developed method of analysis with the official method ( uv-vis-spectrophotometry ) of analysis using the standard addition method. It shows that there was no significant difference via the use of t- test at α=0.05(95% confidence) between the two methods. Therefore the newly developed method can be used of as an alternative method for the analysis of ciprofloxacin.