ISSN: 1813-0410

Author : Khadhair Merza, Thamer

Genetic Discrimination among 21 Corn (Zeay mays L.) Varieties through Total Soluble Seed Protein Profile

Thamer Khadhair Merza; Attyaf Jmeel Thamir; Nidhal Abdul Hussein Al-Badeiry

journal of kerbala university, 2014, Volume 10, Issue 0, Pages 168-175

This study was conducted to investigate the ability of total soluble seed protein to discriminate among 21 of maize genotypes through using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). According to SDS analysis , a total of 118 amplified bands were obtained ranging in their molecular size 18-86KDa. Five out of main nine bands were polymorphic and four monomorphic with an average of polymorphism reaches 55.5%.
Phylogenetic tree divided 21 of corn genotypes between two major groups each of them divided in to two subgroups .The first main group included 13 genotypes , while the second main group included eight genotypes .
The ability of protein profile to give a distinctive pattern for a particular genotype could serve in future of studying corn seed response to different abiotic stress and studying genetic diversity .