Author : Abd,
Study effect of lead acetate on sperms abnormalities and histopathological alternates of testes tissue
journal of kerbala university,
2015, Volume 11, Issue 2, Pages 208-216
This study is relevant in assess the genotoxic effects of lead acetate using sperm abnormalities assay, and study the histopathological changes induced by exposure of lead acetate in testicular tissue of albino male mice, between the treated and the negative control groups. Sixteen mature male mice were used and divided into four groups, the first group kept as a control, the second, third and fourth groups were given18mg/kg b.w of lead acetate daily by oral dosage for one, two and three weeks respectively. The Results showed a statistically significant differences (p < 0.5) represented in increase the number of abnormal sperms in treated animals for one, two and three weeks compared with control group. This results showing a different types of sperm abnormalities as tail less sperm, head less sperm, hook less sperm, amorphous head sperm, pin head sperm, triangle head sperm and eight head sperm. Administration of lead acetate induced enormous generation of Reactive Oxygen Species (ROS). The interaction of some of these (ROS) with the genetic material effect on the differentiation of the spermatogonial cells during spermatogenesis may be responsible for these observations.
As well as diverse histopathological changes were observed in the testis of male mice had been induced by lead acetate for different periods. The histopathological change was represented in relative decrease in spermatogenic activity, , intercellular spaces was appeared between seminiferous tubules and lack of leydig cells number compared with normal state of control group.