ISSN: 1813-0410

Author : A. Al-Waeely, Farah

Molecular detection of some virulence genes of Staphylococus saprophyticus isolated from women with cystitis cases

Farah A. Al-Waeely; Jawad Kadhim AL-khafaji; Zainab Hmood Al-Saadi

journal of kerbala university, Volume 11, Issue 2, Pages 259-267

The study included isolation and identification of uropathogenic Staphylococcus saprophyticus which responsible for cystitis occurrence in young women. A total of 140 mid stream urine sample were taken from women suffering from urinary tract infection that attended to the Hilla general teaching hospital during a period from November 2014 to February 2015 . The patients age ranged from 16 to 55 years. Only 14 isolates were identified as Staphylococcus saprophyticus which have been diagnosed by using selective medium, biochemical tests and by using molecular assays. Molecular detection of Ubiquitous uro-adherence factor A was done by using specific PCR primer . This marker was shown to be present in all isolates of this bacterium ,while the molecular detection of the capsular polysaccharides gene capD has shown that only one isolate (7%) has this gene and gave positive result for this primer. Moreover, Autolysin-adhesin surface protein Aas was also detected in S.saprophyticus . It was shown that all isolates 14(100%) gave positive result for this gene.The collagen binding protein gene sdrI was also investigated in this bacteria and this study has shown that only one isolate possess this gene.In addition to that , the surface associated protein gene of S.saprophyticus Ssp was detected in this study by using specific primer in 12(85%) of isolates , this gene is responsible for lipase encoding .