Author : Flaih Abd-Al-Hussan, Ghofran
journal of kerbala university,
Volume 12, Issue 0, Pages 186-192
Bacteria was isolated from soil sample of various automobile work shop of five different areas in Iraq.About 50 isolate suspected to belong to genus Bacillus.All these isolates were subjected to microscopic examination and cultural characteristics about 42 isolate belonged to genus B.subtilis. Crude biosurfactants produced by the selected isolates were extracted by acid precipitation followed by partially purified with dichloromethane.Biosurfactant has been purified from B.subtilis S4 by two purification steps included Ion exchange chromatography using DEAE- Cellulose followed by Gel filtration chromatography using Sepharose-6B.The the characteristics of the purification compouned were studied . Results showed that the molecular weight of biosurfactant produced by B.subtilis S4 ranging between (1036-1058 Da) with the HPLC-MS System .