Author : J. Al- Sharrifi, Shaymaa
In-vitro study the effects of sickle cell disease on phagocytic activity of white blood cells among adult male in Basra city
journal of kerbala university,
2016, Volume 12, Issue 0, Pages 36-41
Background: sickle cell disease (SCD) is an inherited disorder of hemoglobin synthesis that is associated with a significant morbidity and mortality. The patho physiology of the disease involves abnormalities not just in red blood cells but also white blood cells (leukocytes) functions.
Objective: this study was tried to identify the effect of sickle cell disease SCD on the phagocytic activity of non- isolated leukocytes from the whole blood, to mirror the in vivo stimulation of leukocytes and the effect of SCD on their phagocytic activity.
Materials and methods: this study involved (56) adult male, aged (18-30) years, subdivided into two groups; case group that consist of (26) patient with sickle hemoglobin phenotype (SS) in a steady state at the time of the study and control group that consist of (30) person with normal hemoglobin phenotype (AA). Hemoglobin electrophoresis and leukocytes count were estimated for both groups. The samples of blood were prepared for chemiluminescence (CL) measurements. Luminol was used to increase the amount of measurable light emitted due to liberation of oxygen metabolites during phagocytosis.
Results: Show a significant decrease in the phagocytic activity of SCD patients (P ˂ 0.0001) in comparison with normal volunteers