Author : K. Arrack, Jawad
Protective Role of Pomegranate peel extract on the Pituitary gland of adult male rabbits treated with CCL4
journal of kerbala university,
2014, Volume 10, Issue 3, Pages 83-98
This study was carried out to investigate the protective role of pomegranate peel extract for relieving the harmful effects of carbon tetrachloride on the pituitary gland function in adult male rabbits. Twenty four adult male rabbits were used in this study and divided randomly into four equal groups. Rabbits of the 1st group were received 1 ml of orally distal water once a day and olive oil 1/2 ml /kg B.W. intraperitoneal injection [I.P] twice a week as a control group. The second group were treated I.P with 500 mg / kg B.W. of CCL4 mixing with equal volume of olive oil (o.5 ml/kg B.W.) twice a week (group T1). The third group were received orally pomegranate peel extract (100 mg/kg B.W) once a day and olive oil 1/2 ml /kg B.W. I.P twice a week ( group T2). The fourth group were received pomegranate peel extract (100 mg/kg B.W) once a day and treated I.P with 500 mg / kg B.W. of CCL4 mixing with equal volume of olive oil (o.5 ml/kg B.W.) twice a week (group T3). Blood samples were collected at on days (0, 14, 28.42 and 56) for measuring LH, FSH concentrations, total serum protein after treatment. Results showed a significant (P<0.05) decrease in total serum protein in T1 group compared with control group. While T2 group showed a significant (P<0.05) increase in these parameter as compared with the control and T1 group at the end of treatment period. T1 group indicated i a significant (P<0.05) increase in serum LH at(zero and 14 day) and FSH at( from zero time to the 28 day) . These levels, however, were significantly decreased in T1 group compared to what we observed in the control ones at the end of the experiment period. T2 group also showed a significant (P<0.05) increase in serum FSH at (24 day to the 56day of experiment) and LH concentration (at 56 day) compared to control group. Histological study was showed sever congestion of blood vessel and necrotic area in section of anterior pituitary gland in group treated with CCL4, while demonstrate moderate congestion in T3 group.