Author : Ali Hadi, Yasameen
journal of kerbala university,
Volume 13, Issue 2, Pages 140-147
This work aimed to study the cytotoxic effect of Ethanolic Olive Leaf extract isolated from local oleo eurepea plant on affected living prostate cancer cells towards apoptosis by using high content screening assay (HCS). To achieve this goal, we examine and in vitro evaluate the cytotoxic effect of the Ethanolic Olive leaves extract (OLE) at different concentrations (100, 50, 25, 12.5, 6.25 µg/ml) in a triplicate dilutions in one culture models (prostate cancer cells PC3) and exposure time of treatment (24 hrs) by high content screening. The study found that olive leaves extract at (50 and100 µg/ml) when incubated with these cells, olive leaves extract mediated lead to significant changes(171%,126%) respectively in mitochondrial membrane potential (MMP) and significant increasing (43.61%,70.15%) of releasing cytochrome C into cystol, also olive leaves extract at 100 µg/ml were found effective and appeared to cause the significant induction(74.77%) of nucleus size and morphology and also significant result (81.71%) in cell membrane permeability and viable cell count (significant value P<0.05) suggesting that olive leaves extract appeared as a potential PC3 inhibitor when compared to doxorubicin(20µM) as positive control while other doses marked not any significant effect.