Author : Azeez Addoos, Salman
Association between gene polymorphism in programmed cell death 1 (PD1.1) and susceptibility for rheumatoid arthritis in an Iraqi patients? Case control study.
journal of kerbala university,
Volume 13, Issue 4, Pages 19-25
Programmed cell death-1 (PDCD1) gene is a negative regulator of T-cell to maintain peripheral tolerance and is a key molecule in the development of autoimmune diseases. Although gene polymorphism in PDCD1 was reported to be associated with rheumatoid arthritis (RA), replication studies later on showed conflicting results. This study aimed to determine whether SNP PD1.1 in PDCD1 gene is associated with susceptibility for RA. Methods: Clinical diagnosis of the RA patients was confirmed by the Rheumatology Center of Al Sadder Medical Teaching Hospital in Al-Najaf Al-Ashraf city. Genomic DNA was extracted from the whole blood samples using commercial kit (FavorPrep™ Blood Genomic DNA Extraction Mini Kit). Programmed cell death-1 (PD1.1 G/A SNP) genotyping was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to 60 RA patients and 70 non-autoimmune control group. The genotype and allele frequencies of these SNP were analyzed by statistical tests. Results: There were no significant differences in alleles and genotypes of PD1.1 SNP in PDCD-1 gene between RA cases and control group. Conclusions: PD-1.1 polymorphism in the PDCD1 gene was not associated neither with susceptibility for RA nor with clinical course in rheumatoid arthritis patients (at least in an Iraqi population particularly in AL-Najaf city).